Protein samples were extracted from the cerebellum tissues and quantified using commercially available kits (Beyotime institute of biotechnology, P.R. China). Detailed treatment methods were performed as previously described (Zhang et al. 2019 (link)). MTF1 (1:700) antibody was purchased from Nan-Jing AnYan Biotechnology Co., Ltd; SepSecS (1:500) antibody was synthesized from Jin-Long Li Lab; GPX4 (1:800) antibody was purchased from Abclonal, USA; β-actin (1:1000, Beijing Biosynthesis Biotechnology Co., Ltd); and secondary antibody against rabbit IgG was purchased from Santa Cruz, CA.
Gpx4 1 800 antibody
Manufactured by ABclonal
Sourced in United States
The GPX4 (1:800) antibody is a laboratory equipment product manufactured by ABclonal. It is an immunochemical reagent used for the detection and quantification of the GPX4 protein in biological samples through various analytical techniques, such as Western blotting and immunohistochemistry. The antibody has a recommended dilution of 1:800 for such applications.
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Lab products found in correlation
2 protocols using gpx4 1 800 antibody
1
Cerebellum Tissue Protein Extraction and Quantification
2
Quantifying Cerebellar Protein Levels
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Proteins were extracted from tissues of cerebellum and then quantified through use of commercially available kits (Beyotime Institute of Biotechnology, P.R. China). Detailed methods of treatment were adopted as specified in Xue et al. (Zhao et al., 2020c (link); Talukder et al., 2021 ). The MTF1 (1:700) antibody was purchased from Nan-Jing AnYan Biotechnology Co., Ltd; the Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SepSecS) (1:500) antibody was synthesized from Jinlong Li Lab; the Gpx4 (1:800) antibody was purchased from Abclonal, USA; the β-actin (1:1000) was purchased from Beijing Biosynthesis Biotechnology Co., Ltd; the secondary antibody against rabbit immunoglobulin G (IgG) (1:3000) was purchased from Santa Cruz, CA, USA.
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