K. alvarezii lectins KAA-1 and KAA-2 (previously declared as ECA-1 and ECA-2) and Eucheuma serra lectin ESA-2 were prepared as described previously (Kawakubo et al. 1997 (link), 1999 (link)) and kept at −20 °C until use. The cultivated specimen of K. alvarezii was stored in RNAlater (Life Technologies, CA, USA) at −20 °C until RNA extraction. A vector pGEM-T Easy (Promega, WI, USA) and E. coli strain DH-5α were used for subcloning PCR amplified products. An expression vector pET-28a(+) (Merck, Darmstadt, Germany) and an E. coli strain SHuffle T7 Express (New England Biolabs, MA, USA) were used for recombinant lectin preparation. Pyridylaminated (PA)-sugars were purchased from Takara Bio (Tokyo, Japan), except PA-derivatives of oligomannosides including mannotriose (Manα1-6(Manα1-3)Man-OH) and mannopentaose (Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man-OH) which were previously prepared (Hori et al. 2007 (link)). A recombinant glycosylated HIV-1 IIIB gp120 (baculovirus) was purchased from ImmunoDiagnostics (MA, USA). Jurkat cells and T lymphocyte-derived cells were propagated in RPMI 1640 medium (Life Technologies) supplemented with 10 % fetal calf serum (Nichirei Biosciences, Tokyo, Japan).
Pyridylaminated pa sugars
Manufactured by Takara Bio
Sourced in Japan
Pyridylaminated (PA)-sugars are a type of glycan analysis reagent used for the structural analysis of glycans. These compounds are obtained by reacting sugars with 2-aminopyridine under reducing conditions, resulting in the attachment of a pyridylamino group to the reducing end of the sugar. PA-sugars can be used in various analytical techniques, such as high-performance liquid chromatography (HPLC) and mass spectrometry, to facilitate the separation, detection, and identification of glycan structures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pyridylaminated pa sugars
1
Purification and characterization of marine lectins
Check if the same lab product or an alternative is used in the 5 most similar protocols
K. alvarezii lectins KAA-1 and KAA-2 (previously declared as ECA-1 and ECA-2) and Eucheuma serra lectin ESA-2 were prepared as described previously (Kawakubo et al. 1997 (link), 1999 (link)) and kept at −20 °C until use. The cultivated specimen of K. alvarezii was stored in RNAlater (Life Technologies, CA, USA) at −20 °C until RNA extraction. A vector pGEM-T Easy (Promega, WI, USA) and E. coli strain DH-5α were used for subcloning PCR amplified products. An expression vector pET-28a(+) (Merck, Darmstadt, Germany) and an E. coli strain SHuffle T7 Express (New England Biolabs, MA, USA) were used for recombinant lectin preparation. Pyridylaminated (PA)-sugars were purchased from Takara Bio (Tokyo, Japan), except PA-derivatives of oligomannosides including mannotriose (Manα1-6(Manα1-3)Man-OH) and mannopentaose (Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man-OH) which were previously prepared (Hori et al. 2007 (link)). A recombinant glycosylated HIV-1 IIIB gp120 (baculovirus) was purchased from ImmunoDiagnostics (MA, USA). Jurkat cells and T lymphocyte-derived cells were propagated in RPMI 1640 medium (Life Technologies) supplemented with 10 % fetal calf serum (Nichirei Biosciences, Tokyo, Japan).
+ Expand
K. alvarezii lectins KAA-1 and KAA-2 (previously declared as ECA-1 and ECA-2) and Eucheuma serra lectin ESA-2 were prepared as described previously (Kawakubo et al. 1997 (link), 1999 (link)) and kept at −20 °C until use. The cultivated specimen of K. alvarezii was stored in RNAlater (Life Technologies, CA, USA) at −20 °C until RNA extraction. A vector pGEM-T Easy (Promega, WI, USA) and E. coli strain DH-5α were used for subcloning PCR amplified products. An expression vector pET-28a(+) (Merck, Darmstadt, Germany) and an E. coli strain SHuffle T7 Express (New England Biolabs, MA, USA) were used for recombinant lectin preparation. Pyridylaminated (PA)-sugars were purchased from Takara Bio (Tokyo, Japan), except PA-derivatives of oligomannosides including mannotriose (Manα1-6(Manα1-3)Man-OH) and mannopentaose (Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man-OH) which were previously prepared (Hori et al. 2007 (link)). A recombinant glycosylated HIV-1 IIIB gp120 (baculovirus) was purchased from ImmunoDiagnostics (MA, USA). Jurkat cells and T lymphocyte-derived cells were propagated in RPMI 1640 medium (Life Technologies) supplemented with 10 % fetal calf serum (Nichirei Biosciences, Tokyo, Japan).
2
Lectin Purification and Glycan Binding Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
K. alvarezii lectins KAA-1 and KAA-2 (previously declared as ECA-1 and ECA-2) and Eucheuma serra lectin ESA-2 were prepared as described previously (Kawakubo et al. 1997 (link), 1999 (link)) and kept at −20 °C until use. The cultivated specimen of K. alvarezii was stored in RNAlater (Life Technologies, CA, USA) at −20 °C until RNA extraction. A vector pGEM-T Easy (Promega, WI, USA) and E. coli strain DH-5α were used for subcloning PCR-amplified products. An expression vector pET-28a(+) (Merck, Darmstadt, Germany) and an E. coli strain SHuffle T7 Express (New England Biolabs, MA, USA) were used for recombinant lectin preparation. Pyridylaminated (PA)-sugars were purchased from Takara Bio (Tokyo, Japan), except PA-derivatives of oligomannosides including mannotriose (Manα1-6(Manα1-3)Man-OH) and mannopentaose (Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man-OH) which were previously prepared (Hori et al. 2007 (link)). A recombinant glycosylated HIV-1 IIIB gp120 (baculovirus) was purchased from ImmunoDiagnostics (MA, USA). Jurkat cells and T lymphocyte-derived cells were propagated in RPMI 1640 medium (Life Technologies) supplemented with 10 % fetal calf serum (Nichirei Biosciences, Tokyo, Japan).
+ Expand
K. alvarezii lectins KAA-1 and KAA-2 (previously declared as ECA-1 and ECA-2) and Eucheuma serra lectin ESA-2 were prepared as described previously (Kawakubo et al. 1997 (link), 1999 (link)) and kept at −20 °C until use. The cultivated specimen of K. alvarezii was stored in RNAlater (Life Technologies, CA, USA) at −20 °C until RNA extraction. A vector pGEM-T Easy (Promega, WI, USA) and E. coli strain DH-5α were used for subcloning PCR-amplified products. An expression vector pET-28a(+) (Merck, Darmstadt, Germany) and an E. coli strain SHuffle T7 Express (New England Biolabs, MA, USA) were used for recombinant lectin preparation. Pyridylaminated (PA)-sugars were purchased from Takara Bio (Tokyo, Japan), except PA-derivatives of oligomannosides including mannotriose (Manα1-6(Manα1-3)Man-OH) and mannopentaose (Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man-OH) which were previously prepared (Hori et al. 2007 (link)). A recombinant glycosylated HIV-1 IIIB gp120 (baculovirus) was purchased from ImmunoDiagnostics (MA, USA). Jurkat cells and T lymphocyte-derived cells were propagated in RPMI 1640 medium (Life Technologies) supplemented with 10 % fetal calf serum (Nichirei Biosciences, Tokyo, Japan).
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