Human studies were conducted with informed consent under IRB-approved protocol at Washington University School of Medicine. Unfractionated whole blood and bone marrow aspirates were collected from patients with PDAC and healthy controls in EDTA vacuum tubes (BD Bioscience) and underwent red blood cell (RBC) lysis per manufacturer’s instructions (BioLegend). Resected tumour or normal pancreas from organ donors without malignancy were processed at time of collection. Baseline and post-treatment tumour biopsies from a clinical trial were processed as previously described.19 (link) Tissue was allocated for cryopreservation, fixation or enzymatically digested to create a single cell suspension using GentleMACS (Miltenyi).
Edta vacuum tubes
Manufactured by BD
Sourced in United Kingdom
EDTA vacuum tubes are laboratory equipment used for the collection of blood samples. They contain the anticoagulant Ethylenediaminetetraacetic acid (EDTA), which prevents the blood from clotting during the collection and transportation process.
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Lab products found in correlation
2 protocols using edta vacuum tubes
1
Tissue Processing for Single-Cell Profiling
2
Plasma and Tissue Profiling of HCC
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A study population of ten HCC patients was enrolled at the Sun Yat-sen University Cancer Center (Guangzhou, China). The diagnosis of HCC was confirmed by histology. Pre-surgical blood samples from the HCC patients and healthy subjects were collected in EDTA-vacuum tubes (BD, Polymouth, UK), and plasma was isolated within 30 min. The blood collection tubes were centrifuged at 500 g for 10 min at 4°C, the supernatant layer was transferred to new RNase-free tubes, and the samples were further centrifuged at 1800 g for 10 min at 4°C to prepare plasma without cell debris. Corresponding pairs of primary HCC tissues and the adjacent non-tumor tissues were obtained from the patients undergoing resection at Sun Yat-sen University Cancer Center. The tissues were immediately treated with RNA-later (Ambion, Austin, TX), and stored at -80°C before use. All study subjects provided written consent, and the present study was approved with the statement (2012H007) by the Human Research Ethics Review Board of Beijing Institute of Genomics, CAS.
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