Hs4000 sequencer

Manufactured by Illumina

Sourced in United States

The HS4000 Illumina sequencer is a high-throughput genetic sequencing instrument. It is designed to generate DNA sequence data by performing Next-Generation Sequencing (NGS) on biological samples. The HS4000 utilizes Illumina's proprietary sequencing-by-synthesis technology to produce reliable and accurate genomic data.

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Lab products found in correlation

Rna seq libraries, by Illumina (2 mentions) Truseq stranded mrna kit, by Illumina (2 mentions)

2 protocols using hs4000 sequencer

Illumina and Nanopore RNA-Seq of Female Flowers

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Six Illumina RNA-Seq libraries (one for each organ) were constructed from 500 ng total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, United States), which allows for mRNA strand orientation (the orientation of sequences relative to the antisense strand is recorded). Each library was sequenced using 151 bp paired-end read chemistry on a HS4000 Illumina sequencer (Illumina, San Diego, CA, United States).
One Nanopore cDNA library was also prepared from entire female flower RNA. The cDNA library was obtained from 50 ng RNA following the Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd., Oxford, United Kingdom) protocol “cDNA-PCR Sequencing (SQK-PCS108)” with a 14 cycle PCR (6 min for elongation time). ONT adapters were ligated to 190 ng of resulting cDNA. The Nanopore library was sequenced using a MinION Mk1b and R9.4.1 flowcells.

Mishra B., Ulaszewski B., Meger J., Aury J.M., Bodénès C., Lesur-Kupin I., Pfenninger M., Da Silva C., Gupta D.K., Guichoux E., Heer K., Lalanne C., Labadie K., Opgenoorth L., Ploch S., Le Provost G., Salse J., Scotti I., Wötzel S., Plomion C., Burczyk J, & Thines M. (2022). A Chromosome-Level Genome Assembly of the European Beech (Fagus sylvatica) Reveals Anomalies for Organelle DNA Integration, Repeat Content and Distribution of SNPs. Frontiers in Genetics, 12, 691058.

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Illumina and Nanopore Transcriptome Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Six Illumina RNA-Seq libraries (one for each organ) were constructed from 500ng total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows for mRNA strand orientation (the orientation of sequences relative to the antisense strand is recorded). Each library was sequenced using 151 bp paired end reads chemistry on a HS4000 Illumina sequencer.
One Nanopore cDNA library was also prepared from entire female flowers RNA. The cDNA library was obtained from 50 ng RNA according to the Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd, Oxford, UK) protocol "cDNA-PCR Sequencing (SQK-PCS108)" with a 14 cycles PCR (6 minutes for elongation time). ONT adapters were ligated to 190 ng of cDNA. The Nanopore library was sequenced using a MinION Mk1b with R9.4.1 flowcells.

Mishra B., Ulaszewski B., Meger J., Aury J., Bodénès C., Ojeda D.I., Pfenninger M., Silva C.D., Gupta D., Guichoux E., Heer K., Lalanne C., Labadie K., Opgenoorth L., Ploch S., Provost G.L., Salse J., Schueler S., Wötzel S., Plomion C., Burczyk J., & Thines M. (2021). A chromosome-level genome assembly of the European Beech (Fagus sylvatica) reveals anomalies for organelle DNA integration, repeat content and distribution of SNPs.

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