Recombinant mouse IL-1β (10 ng/ml, R&D Systems), LPS (Sigma-Aldrich; catalog #L4391), TAK-242 (Sigma-Aldrich; catalog #614316), minocycline (Sigma-Aldrich; catalog #M9511-1G), TLR2 antibody (BioLegend; catalog #121801), BAY 11-7082 (Cayman Chemical; catalog #10010266), (+)-sodium L-ascorbate (vitamin C sodium salt; Sigma-Aldrich; catalog #A7631), ascorbyl palmitate (Sigma-Aldrich; 76183), ATP (Sigma-Aldrich; catalog #A26209), apyrase (Sigma-Aldrich; catalog #A6410).
Tlr2 antibody
Manufactured by BioLegend
Sourced in United States
The TLR2 antibody is a laboratory reagent used for the detection and analysis of the Toll-like receptor 2 (TLR2) protein. TLR2 is a pattern recognition receptor that plays a crucial role in the innate immune response by recognizing a variety of pathogen-associated molecular patterns (PAMPs). The TLR2 antibody can be utilized in various immunological and cell biology applications, such as flow cytometry, Western blotting, and immunohistochemistry, to study the expression and function of TLR2 in different cell types and biological samples.
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Lab products found in correlation
Sodium l ascorbate, by Merck Group (1 mentions)
Recombinant mouse il 1β, by R&D Systems (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Tak 242, by Merck Group (1 mentions)
Apyrase, by Merck Group (1 mentions)
Ascorbyl palmitate, by Merck Group (1 mentions)
Minocycline, by Merck Group (1 mentions)
Bay 11 7082, by Cayman Chemical (1 mentions)
2 protocols using tlr2 antibody
1
Inflammatory Response Modulation Assay
2
Isolation and Culture of Mixed Glial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Primary mixed glial cultures were prepared as previously described [24 (link)]. Briefly, mixed glial cultures were prepared from postnatal day 1 WT or TLR2 KO mice. After removing the meninges from the cerebral hemisphere, the tissue was dissociated into a single-cell suspension by gentle repetitive pipetting. The cells were cultured in DMEM supplemented with 10 mM HEPES, 10% FBS, 2 mM L-glutamine, and 1X antibiotic/antimycotic in 75 cm2 flasks at 37 °C in a 5% CO2 incubator. The medium was changed every 5 days. After 2 weeks, the microglia were removed by treating the cells with 100 mM L-leucine methyl ester for 60 min, harvested using trypsinization (0.25% trypsin, 0.02% EDTA), and seeded in 6-well dishes. To block hemin-induced TLR2 activation, the cells were preincubated with 50 μg/ml of TLR2 antibody (Biolegend, San Jose, CA, USA).
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