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Malachite green phosphatase assay kit

Manufactured by Merck Group
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The Malachite green phosphatase assay kit is a colorimetric assay designed to quantify phosphatase enzyme activity. The kit utilizes malachite green, a dye that forms a complex with free phosphate ions, resulting in a color change that can be measured spectrophotometrically. This provides a simple and efficient method for determining the activity of various phosphatase enzymes.

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Lab products found in correlation

Sphingosine 1 phosphate (s1p), by MyBioSource (1 mentions)

Spelling variants of this product

Malachite green (83 citations) Phosphatases (7 citations) Malachite Green assay (5 citations) Malachite Green Kit (2 citations)

4 protocols using malachite green phosphatase assay kit

1

Lipid Signaling in Cardioprotection

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The concentration of intracardiac and cardiomyocyte acid ceramidase concentrations (USBiological, MA, USA) and S1P concentrations (MyBioSource, San Diego, CA, USA) were determined using an ELISA kit. PP2A activity was evaluated after immunoprecipitation of the catalytic (C) subunit of PP2A using a malachite green phosphatase assay kit (Millipore, Billerica, MA, USA). We also measured intracardiac ceramide species with authentic standards (Sigma-Aldrich, St. Louis, MO, USA, Avanti Polar Lipids, Alabaster, AL), ceramide-nonhydroxy fatty acid conjugated to sphingosine (CNS), and ceramide-nonhydroxy fatty acid conjugated to dihydrosphingosine (CNDS) employing UPLC-MS/MC according to the methods previously described [18 (link)].
Kim Y., Lim J.H., Kim E.N., Hong Y.A., Park H.J., Chung S., Choi B.S., Kim Y.S., Park J.Y., Kim H.W, & Park C.W. (2022). Adiponectin receptor agonist ameliorates cardiac lipotoxicity via enhancing ceramide metabolism in type 2 diabetic mice. Cell Death & Disease, 13(3), 282.
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2

Evaluating PP2A Phosphatase Activity

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PP2A activity was evaluated after immunoprecipitation of the catalytic (C) subunit of PP2A (PP2A-C) using a malachite green phosphatase assay kit (Millipore, Billerica, MA) or by measuring phosphorylation of tyrosine (Tyr) 307 to total PP2A (p-PP2ATyr307:PP2A) by immunoblot.
Bharath L.P., Ruan T., Li Y., Ravindran A., Wan X., Nhan J.K., Walker M.L., Deeter L., Goodrich R., Johnson E., Munday D., Mueller R., Kunz D., Jones D., Reese V., Summers S.A., Babu P.V., Holland W.L., Zhang Q.J., Abel E.D, & Symons J.D. (2015). Ceramide-Initiated Protein Phosphatase 2A Activation Contributes to Arterial Dysfunction In Vivo. Diabetes, 64(11), 3914-3926.
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3

Evaluating PP2A Activity via Immunoprecipitation

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PP2A activity was evaluated after immunoprecipitation utilizing a malachite green phosphatase assay kit as per the manufacturer’s instructions (EMD Millipore).
Eil R., Vodnala S.K., Clever D., Klebanoff C.A., Sukumar M., Pan J.H., Palmer D.C., Gros A., Yamamoto T.N., Patel S.J., Guittard G.C., Yu Z., Carbonaro V., Okkenhaug K., Schrump D.S., Linehan W.M., Roychoudhuri R, & Restifo N.P. (2016). Ionic immune suppression within the tumour microenvironment limits T cell effector function. Nature, 537(7621), 539-543.
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4

Evaluating PP2A Activity via Immunoprecipitation

Check if the same lab product or an alternative is used in the 5 most similar protocols
PP2A activity was evaluated after immunoprecipitation utilizing a malachite green phosphatase assay kit as per the manufacturer’s instructions (EMD Millipore).
Eil R., Vodnala S.K., Clever D., Klebanoff C.A., Sukumar M., Pan J.H., Palmer D.C., Gros A., Yamamoto T.N., Patel S.J., Guittard G.C., Yu Z., Carbonaro V., Okkenhaug K., Schrump D.S., Linehan W.M., Roychoudhuri R, & Restifo N.P. (2016). Ionic immune suppression within the tumour microenvironment limits T cell effector function. Nature, 537(7621), 539-543.
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