Samples were fixed in 2.5% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in 0.1 M cacodylate buffer at pH 7.4 for 12 h, post-fixed in 1% osmium tetroxide for 60 min using the same buffer. Dehydrated samples were embedded in Spurr (Electron Microscopy Sciences). Ultrathin sections (60–70 nm) were cut and mounted on 150 mesh copper grids (Electron Microscopy Sciences). Unstained samples were observed with Talos F200S G2 transmission electron microscope (Thermo Fisher Scientific, Waltham, MA, USA).
Talos f200s g2 transmission electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Talos F200S G2 is a transmission electron microscope (TEM) designed and manufactured by Thermo Fisher Scientific. It is a high-performance instrument that provides advanced imaging and analytical capabilities for materials science and life science research. The Talos F200S G2 enables the observation and analysis of samples at the nanoscale level.
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2 protocols using talos f200s g2 transmission electron microscope
1
Electron Microscopy Sample Preparation
2
Ultrastructural Analysis of Fibroblasts
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Fibroblasts were physically scraped from the plastic substrate, centrifuged, and fixed in 2.5% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, United States) dissolved in 0.1 M cacodylate buffer at pH 7.4 for 12 h. Post-fixation was performed for 60 min in 1% osmium tetroxide using the same buffer. Samples were dehydrated with increasing ethanol concentration up to 100% and embedded in Epon 812 (Electron Microscopy Sciences). Ultrathin sections (60 nm) were cut and mounted on 150 mesh copper grids (Electron Microscopy Sciences). Unstained samples were observed with Talos F200S G2 transmission electron microscope (Thermo Fisher Scientific).
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