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RNF125 is a lab equipment product from Thermo Fisher Scientific. It functions as a protein that is involved in the regulation of RNA metabolism and cellular processes. The core function of RNF125 is to act as an E3 ubiquitin-protein ligase, which is responsible for the ubiquitination of target proteins.

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Lab products found in correlation

Plko 1 vector, by Thermo Fisher Scientific (2 mentions) Hnf4a, by Thermo Fisher Scientific (2 mentions) Sox10, by Thermo Fisher Scientific (2 mentions) Flag tag (flag), by Merck Group (2 mentions) V5 tag, by Thermo Fisher Scientific (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Brafi plx4032, by Selleck Chemicals (2 mentions) Egfri gefitinib zd1839, by Selleck Chemicals (2 mentions) Jaki azd1480, by Selleck Chemicals (2 mentions) Jaki pyridone 6, by Merck Group (2 mentions) Stat3, by Santa Cruz Biotechnology (2 mentions)

4 protocols using rnf125

1

Silencing key regulators via shRNA

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pLKO.1 vectors targeting RNF125, JAK1, EGFR, HNF4a, SOX10, and MITF were purchased from Thermo Scientific. After transduction of shRNA lentivirus, silencing efficiency was validated from cell lysates of stable clones using the appropriate antibodies or by qPCR analysis of mRNA using the primers shown in Table S4.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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2

Silencing key regulators via shRNA

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pLKO.1 vectors targeting RNF125, JAK1, EGFR, HNF4a, SOX10, and MITF were purchased from Thermo Scientific. After transduction of shRNA lentivirus, silencing efficiency was validated from cell lysates of stable clones using the appropriate antibodies or by qPCR analysis of mRNA using the primers shown in Table S4.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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3

Molecular Signaling Pathways Protocol

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The BRAFi PLX4032, the EGFRi gefitinib/ZD1839, and the JAKi AZD1480 were purchased from Selleckchem. The JAKi pyridone 6 was purchased from Millipore. The following antibodies were used: FLAG-tag from Sigma-Aldrich; V5-tag from Life Technology or Bethyl Laboratories; Stat3, β-actin, GFP, and control IgG from Santa Cruz; TYK2 from BD Biosciences; JAK2, JAK3, EGFR, AXL, pStat3, and DDX58/RIG-I from Cell Signaling; GAS6 from R&D Systems; RNF125 from Life Technology, Bethyl Laboratories, AbCam, or Sigma-Aldrich; and JAK1 from Santa Cruz, BD Biosciences, Cell Signaling, or EMD Millipore.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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4

Molecular Signaling Pathways Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The BRAFi PLX4032, the EGFRi gefitinib/ZD1839, and the JAKi AZD1480 were purchased from Selleckchem. The JAKi pyridone 6 was purchased from Millipore. The following antibodies were used: FLAG-tag from Sigma-Aldrich; V5-tag from Life Technology or Bethyl Laboratories; Stat3, β-actin, GFP, and control IgG from Santa Cruz; TYK2 from BD Biosciences; JAK2, JAK3, EGFR, AXL, pStat3, and DDX58/RIG-I from Cell Signaling; GAS6 from R&D Systems; RNF125 from Life Technology, Bethyl Laboratories, AbCam, or Sigma-Aldrich; and JAK1 from Santa Cruz, BD Biosciences, Cell Signaling, or EMD Millipore.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
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