Anti human cd117 phycoerythrin pe

The ovarian cancer tissues were harvested from the mice immunized with the different vaccines at the end of the experiments, and were developed into cell suspension that were used to analyze the CD44⁺CD117⁺CSC population by FCM assay. Briefly, a total of 2 × 10⁵ tumor cells were suspended in PBS and labeled with anti-Human/Mouse CD44 fluorescein isothiocyanate (FITC) 1:100 (eBioscience, CA, USA), and anti-Human CD117 phycoerythrin (PE) 1:20 (eBioscience, CA, USA) antibodies for immunofluorescence detection. Equal number of the cells cultured in stem cell culture medium was analyzed by FCM with Beckman Coulter Cell Quest software [9 (link), 21 (link)].

To detect the CD44⁺CD117⁺ cell and ALDH-positive cell subsets, the CD44 and CD117 antibodies (eBioscience, USA) and the ALDH enzyme assay kit (Invitrogen, Carlsbad, CA, USA) were applied on a flow cytometer (FCM, BD Biosciences, USA) according to the manufacturer's instructions and our previous reports [16 (link), 19 (link)]. Briefly, a total of 2 × 10⁵ tumor cells from tumor tissues harvested from the mice immunized with different vaccines were suspended in PBS and labeled with anti-human/mouse CD44 fluorescein isothiocyanate (FITC) 1 : 100 (eBioscience, CA, USA) and anti-human CD117 phycoerythrin (PE) 1 : 20 (eBioscience, CA, USA) antibodies for immunofluorescence detection of the percentage of CSCs by a flow cytometer (FCM).