CRA-1 was purchased from Kyokuto (Tokyo, Japan). Mouse IgG and anti-human IgE fluorescein isothiocyanate (FITC) antibodies were purchased from Biosources (Burlingame, CA, USA). Anti-mouse IgG FITC antibody was purchased from Jackson ImmunoResearch Laboratories, Inc. (Baltimore, PO, USA). The RPMI-1640 medium, antibiotics, antimycotics, and fetal bovine serum (FBS) were obtained from GIBCO BRL (Gaithersburg, MD, USA). The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega (Madison, WI, USA). The TRIzol reagent, Superscript II reverse transcriptase, and oligo(dT)12–18 primer were purchased from Invitrogen (Carlsbad, CA, USA). The Taq DNA polymerase was purchased from Roche (Mannheim, Germany). The dNTP set was purchased from Amersham Pharmacia Biotech Inc. (Piscataway, NJ, USA). All other reagents, including hydroxyethyl piperazineethanesulfonic acid (HEPES), Fura 2-acetoxymethyl ester (AM), dimethylsulfoxide, histamine, and o-phthalaldehyde (OPA) were purchased from Sigma Chemicals (St. Louis, MO, USA).
3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium inner salt
Manufactured by Promega
Sourced in United States
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt is a chemical compound used in colorimetric assays to measure cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Antimycotics, by Thermo Fisher Scientific (1 mentions)
Histamine, by Merck Group (1 mentions)
Hydroxyethyl piperazine ethane sulfonic acid hepes, by Merck Group (1 mentions)
Anti mouse igg fitc antibody, by Jackson ImmunoResearch (1 mentions)
Oligo dt 12 18 primer, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
O phthalaldehyde, by Merck Group (1 mentions)
Taq dna polymerase, by Roche (1 mentions)
Heparan sulfate, by Merck Group (1 mentions)
Heparin, by Merck Group (1 mentions)
Dynasore, by Merck Group (1 mentions)
Silver nitrate, by Thermo Fisher Scientific (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
7 protocols using 3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium inner salt
1
Evaluating Cellular Responses using MTS Assay
2
Plai Oil Cytotoxicity Evaluation
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Plai essential oil was obtained from Thai China Flavours & Fragrances Industry Co., Ltd. (Bangkok, Thailand). PEO (Mw ~ 100,000) and PLA (Mw ~ 60,000) were acquired from Sigma Aldrich. Dulbecco’s modified Eagle’s medium (DMEM, Gibco®, MD, USA) with 10% fetal bovine serum (FBS, Gibco®), 1 mM sodium pyruvate (Gibco®), and penicillin/streptomycin (Gibco®) were supplied by Life Technologies. In addition, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt was from obtained Promega Corporation. All buffer salts and organic solvents were of analytical grade from Merck.
3
Evaluating Marine Saccharides for Biomedical Applications
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All marine saccharides used in this study were kindly provided by the Glycoscience and Glycoengineering Laboratory at the School of Medicine and Pharmacy of Ocean University of China. Heparin and heparan sulfate (HS) were purchased from Sigma Aldrich (St. Louis, MO, USA) and Iduron (Manchester, UK), respectively. [3-(4, 5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] (MTS) was purchased from Promega Co. (Madison, WI, USA). Dynasore and fluorescein isothiocyanate (FITC)-conjugated anti-RABV N protein antibody were purchased from Sigma Aldrich (St. Louis, MO, USA) and Fujirebio Diagnostics (Malvern, PA, USA), respectively.
4
Phyllanthus emblica Fruit Extract Characterization
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Phyllanthus emblica fruit extract was obtained from Chemipan Corporation Co., Ltd. (Bangkok, Thailand). Polyvinylpyrrolidone K30 (MW~40,000) and PEG (Mw~400) were attained from TCI chemicals (Tokyo, Japan). Silver nitrate was from Alfa Aesar (Kandel, Germany). Folin–Ciocalteu reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Burlington, MA, USA). (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt was obtained from Promega Corporation (Madison, WI, USA). Dulbecco’s modified Eagle’s medium (DMEM, Gibco®) with 10% fetal bovine serum (FBS, Gibco®), 1 mM sodium pyruvate (Gibco®), and penicillin/streptomycin (Gibco®) were supplied by Life Technologies (Carlsbad, CA, USA). Buffer salts and organic solvents were of analytical grade from Merck (Darmstadt, Germany).
5
Recombinant GPx4 Enzyme Assay
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Recombinant GPx4 protein mus musculus (mouse) was obtained from Cloud−Clone Crop. (Wuhan, China). EGCG, genistein and selenium dioxide SeO2 (IV) were purchased from Aladdin (Shanghai, China). H2O2 (30%), cumene hydroperoxide (Cu-OOH), sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), glutathione (GSH), glutathione reductase (GR), sodium citrate (Na3C6H5O7, 99.8%), dimethyl sulfoxide (DMSO) and silver nitrate (AgNO3, 99.0%) were obtained from Sigma-Aldrich (Shanghai, China). MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] was purchased from Promega (Madison, USA). Dulbecco's modified eagle medium (DMEM), fetal calf serum (FBS), penicillin–streptomycin, and phosphate-buffered saline (PBS) were obtained from Gibco (Grand Island, NY, USA). Cell-culture plates and cell-culture dishes were purchased from Corning Incorporated (USA). PC12 cells were provided by Shanghai Cellular Institute of China Scientific Academy. Structure of part chemicals as shown in Table A1. A total glutathione peroxidase assay kit with nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from the Beyotime Institute of Biotechnology (Shanghai, China). The principle of testing is as follows:
6
Dexamethasone and Cell Signaling Pathways
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Dexamethasone, hydrocortisone, rolipram, forskolin, and 1,9-dideoxyforskolin were obtained from Biomol (Plymouth Meeting, PA, USA). Phenazine methosulfate (PMS) and protease inhibitor cocktail for use with mammalian cell and tissue extracts were from Sigma-Aldrich (St. Louis, MO, USA). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] (MTS) was from Promega (Madison, WI, USA). Primary rabbit polyclonal antibodies directed against GR, Bim, Bad, and S112 phospho-Bad were obtained either from Santa Cruz Biotechnology (Santa Cruz, CA, USA), Cell Signaling Technologies (Danvers, MA, USA), Biosource International (Camarillo, CA, USA), or Zymed Laboratories (South San Francisco, CA, USA). The specific antibodies used were: GR, Santa Cruz sc-8992; Bim, Santa Cruz sc-11425 and Zymed 38-6500; Bad, Santa Cruz sc-7869 and Cell Signaling Technologies 9292; phospho-Bad, Santa Cruz sc-7998-R, Cell Signaling Technologies 9291 and Biosource International 44-522. Primary rabbit monoclonal antibody directed against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was obtained from Cell Signaling Technologies. Anti-Rabbit IgG-horseradish peroxidase was obtained from GE Healthcare (Piscataway, NJ, USA).
7
Cytotoxicity Assay of Musk Compounds
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Cultured cells were seeded in 96-well plates at a density of 1 × 104 cells per well. Native musk and synthetic musk ketone (four replicates in each group) were added at varying concentrations. After 24 h of treatment, 20 μl of MTS solution [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; Promega Corporation, Madison, WI, USA] was added to each well, and the plates were incubated for 4 h at 37 °C. Absorbance at 490 nm was measured using a spectrophotometer. The same amount of solvent (ethanol) was used as the control.
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