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Pcold tf expression vector

Manufactured by Takara Bio
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Sourced in Japan

The PCold-TF expression vector is a tool designed for the expression of recombinant proteins in E. coli. It features a cold-shock inducible promoter, which allows for the controlled expression of the target protein at lower temperatures, potentially enhancing the solubility and correct folding of the recombinant protein.

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PCold-TF vector (18 citations) PCold-TF (16 citations) PCold vector (12 citations)

2 protocols using pcold tf expression vector

1

His-fused Protein Expression in E. coli

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To produce the His-fused proteins, the full-length coding sequences were amplified and inserted into cloning sites in the pCold-TF expression vector (TAKARA) and the proteins were then expressed in Escherichia coli DE3 (BL21) cells. The cells were collected and sonicated, and the supernatants containing the corresponding fusion proteins were purified with NI-HA beads (QIAGEN). The eluted proteins were analyzed on a 10% SDS-PAGE gel.
Yang J., Ji C, & Wu Y. (2016). Divergent Transactivation of Maize Storage Protein Zein Genes by the Transcription Factors Opaque2 and OHPs. Genetics, 204(2), 581-591.
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2

Cloning of Mouse C8γ cDNA

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Full-length mouse C8γ cDNA (Gene Accession No. NM_027062.2) was amplified by reverse transcription polymerase chain reaction using total RNA from C57BL/6J mouse liver and cloned into pCold-TF expression vector (TaKaRa Bio, Shiga, Japan). The sequences synthesized by polymerase chain reaction were confirmed by DNA sequencing.
Yamamoto K., Hiromori Y., Matsumaru D., Ishii Y., Takeshita Y., Tsubakihara I., Kimura T., Nagase H, & Nakanishi T. (2020). Tri-substituted organotin compounds, but not retinoic acid, are potent ligands of complement component 8 γ. The Journal of toxicological sciences, 45(9).
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