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Anti rabbit igg control antibody

Manufactured by Santa Cruz Biotechnology
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The Anti-rabbit IgG control antibody is a tool used to validate experimental procedures involving rabbit-derived antibodies. It serves as a control to ensure specificity and consistency in immunoassays and other applications where rabbit antibodies are utilized.

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Lab products found in correlation

Dynabeads protein g, by Santa Cruz Biotechnology (2 mentions) Western lightning plus ecl detection kit, by PerkinElmer (2 mentions) Anti utx antibody, by Santa Cruz Biotechnology (2 mentions) Af2944, by R&D Systems (2 mentions)

2 protocols using anti rabbit igg control antibody

1

Immunoprecipitation and Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were incubated with 5 µg anti-UTX antibody (Bethyl), or anti-rabbit IgG control antibody (Santa Cruz) overnight at 4 °C followed by 2 h of incubation with Dynabeads Protein G for immunoprecipitation. Protein complexes bound to antibody and beads were washed 5 times and eluted with Laemmli sample buffer. Samples were resolved by SDS-PAGE. Protein interaction was analyzed by immunoblotting with anti-PLZF (R&D, AF2944), or anti-JunB primary antibodies, and goat anti-rabbit IgG-HRP secondary antibodies, followed by visualization using the Western Lightning Plus ECL detection kit (PerkinElmer).
Beyaz S., Kim J.H., Pinello L., Xifaras M.E., Hu Y., Huang J., Kerenyi M.A., Das P.P., Barnitz R.A., Herault A., Dogum R., Haining W.N., Yilmaz Ö.H., Passegue E., Yuan G.C., Orkin S.H, & Winau F. (2016). The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nature immunology, 18(2), 184-195.
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2

Immunoprecipitation and Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were incubated with 5 µg anti-UTX antibody (Bethyl), or anti-rabbit IgG control antibody (Santa Cruz) overnight at 4 °C followed by 2 h of incubation with Dynabeads Protein G for immunoprecipitation. Protein complexes bound to antibody and beads were washed 5 times and eluted with Laemmli sample buffer. Samples were resolved by SDS-PAGE. Protein interaction was analyzed by immunoblotting with anti-PLZF (R&D, AF2944), or anti-JunB primary antibodies, and goat anti-rabbit IgG-HRP secondary antibodies, followed by visualization using the Western Lightning Plus ECL detection kit (PerkinElmer).
Beyaz S., Kim J.H., Pinello L., Xifaras M.E., Hu Y., Huang J., Kerenyi M.A., Das P.P., Barnitz R.A., Herault A., Dogum R., Haining W.N., Yilmaz Ö.H., Passegue E., Yuan G.C., Orkin S.H, & Winau F. (2016). The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nature immunology, 18(2), 184-195.
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