10 0 nylon surgical suture

Mice were anesthetized with an intraperitoneal injection of 50 mg/kg Nembutal. The conjunctiva at each quadrant was incised and detached from the sclera. 1 or 2 vortex veins are observed in each quadrant of the mouse eye (Fig 1A). All vortex veins of the right eye were sutured at the surface of the sclera employing 10–0 nylon surgical suture (MANI, INC., Utsunomiya, Tochigi, Japan), aiming to induce vortex vein congestion (Fig 1B and S1 Video). All surgeries were performed by the first author.

When all four vortex veins were ligated in the preliminary experiment, hyphema developed postoperatively and the fundus could not be evaluated. In 1973, Hayreh and Baines performed experimental occlusion of monkey vortex veins by cauterization and reported that cauterizing 3–4 vortex veins caused a high rate of hyphema, while cauterizing 1–2 veins resulted in almost no hyphema [17 (link)]. Therefore, two vortex veins of the left eye were ligated in this study. After making a 2/3 circumferential incision of the conjunctiva around the limbus, control threads (3–0 SILK BRAID; Alfresa Pharma Corp., Osaka, Japan) were placed under the superior rectus, external rectus, and inferior rectus muscles. The conjunctiva at the superotemporal and inferotemporal quadrants was incised and detached from the sclera. Then, the superotemporal and inferotemporal vortex veins were ligated at the surface of the sclera employing 10–0 nylon surgical suture (MANI, INC., Tochigi, Japan), with the aim of inducing vortex vein congestion (S1 Fig). Finally, the control threads were removed, and the conjunctiva was sutured at the original position with 9–0 silk surgical suture (Alcon, Inc., Tokyo, Japan).