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Mirna assay u6 snrna

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The miRNA assay U6 snRNA is a molecular biology tool used to detect and quantify the expression levels of the U6 small nuclear RNA (U6 snRNA) in biological samples. U6 snRNA is a small nuclear RNA component of the spliceosome, a complex that plays a crucial role in the processing of pre-messenger RNA. This assay provides a means to measure the abundance of U6 snRNA, which can be used as an internal control or reference gene for the normalization of miRNA expression data.

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Lab products found in correlation

Rotor gene q pcr cycler, by Qiagen (2 mentions) Mirneasy mini kit, by Qiagen (2 mentions) Taqman mirna assays hsa mir 146a, by Thermo Fisher Scientific (1 mentions)

2 protocols using mirna assay u6 snrna

1

Quantifying miR-146a Expression

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microRNA was isolated using miRNeasy Mini Kit (Qiagen). miR‐146a expression was measured using TaqMan miRNA assay hsa‐miR‐146a (Applied Biosystems) according to the manufacturer's instruction using the Rotor‐Gene Q PCR cycler (Qiagen). U6snRNA (miRNA assay U6 snRNA Applied Biosystems) was used as internal control. Relative expression of miR‐146a was calculated by the 2ΔΔCT method.
Saferding V., Hofmann M., Brunner J.S., Niederreiter B., Timmen M., Magilnick N., Hayer S., Heller G., Steiner G., Stange R., Boldin M., Schabbauer G., Weigl M., Hackl M., Grillari J., Smolen J.S, & Blüml S. (2020). microRNA‐146a controls age‐related bone loss. Aging Cell, 19(11), e13244.
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2

miR-146a Expression Analysis

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Micro RNA was isolated using miRNeasy Mini Kit (Qiagen). MiR-146a expression was measured using TaqMan miRNA Assays hsa-miR-146a (Applied Biosystems) according to the manufacturer's instruction using the Rotor-Gene Q PCR cycler (Qiagen). U6snRNA (miRNA assay U6 snRNA Applied Biosystems) was used as internal control. Relative expression of miR-146a was calculated by the 2ΔΔCT method.
Saferding V., Hofmann M., Brunner J., Niederreiter B., Timmen M., Magilnick N., Hayer S., Heller G., Steiner G., Stange R., Boldin M.P., Schabbauer G., Weigl M., Hackl M., Grillari J., Smolen J.S., & Blüml S. (2020). MiR-146a controls age related bone loss.
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