Hartman s fixative solution

Manufactured by Merck Group

Sourced in United States

Hartman's fixative solution is a laboratory reagent used to preserve and fix biological samples for microscopic examination. It is a formaldehyde-based solution that helps maintain the structural integrity of cells and tissues. The core function of Hartman's fixative is to ensure the proper preservation of samples for further analysis and study.

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Lab products found in correlation

Nanozoomer xr digital slide scanner, by Hamamatsu Photonics (1 mentions) Streptavidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Rnascope fluorescent multiplex kit, by Advanced Cell Diagnostics (1 mentions) C57bl 6j wt mice, by Jackson ImmunoResearch (1 mentions) Biotinylated pna, by Vector Laboratories (1 mentions) Bz x810 fluorescent microscope, by Keyence (1 mentions)

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Hartman’s fixative (14 citations)

3 protocols using hartman s fixative solution

Retinal Morphology Analysis in Ocular Models

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Oriented ocular globes were fixed for at least 24 h in Hartman’s fixative solution (Sigma-Aldrich, St. Louis, MI, USA) and embedded in paraffin. Five-µm sagittal sections containing optic nerve (ON) were performed and stained with hematoxylin and eosin (HE). HE sections from 3 consecutive slides were examined by light microscopy at a magnification of 20× and color images were obtained using a NanoZoomer-XR Digital slide scanner (Hamamatsu Photonics, Hamamatsu city, Japan). Images were analyzed using NDP View software. The outer nuclear layer (ONL thickness) in the LID model or the total retinal thickness (from the internal limiting membrane to the retinal pigment epithelium) and the outer retinal thickness (from the outer plexiform layer to the retinal pigment epithelium) in the MNU rat model were measured every 500 µm from the ON to the inferior and the superior ciliary processes. Thickness profiles along the retina were generated by averaging, for each distance, the values obtained for all eyes, as previously described [30 (link)] and values are expressed as mean ± sem. The photoreceptor ratio was calculated as the percentage of outer retinal thickness/total retinal thickness, as previously described [40 (link)].

Bigot K., Gondouin P., Bénard R., Montagne P., Youale J., Piazza M., Picard E., Bordet T, & Behar-Cohen F. (2020). Transferrin Non-Viral Gene Therapy for Treatment of Retinal Degeneration. Pharmaceutics, 12(9), 836.

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In-situ Analysis of Phosphoinositide Kinases

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WT C57BL/6J mice approximately one yr of age (The Jackson Laboratory, Bar Harbor, ME) were euthanized in a CO₂ chamber prior to enucleation. Eyes were fixed in Hartman’s Fixative Solution (Sigma-Aldrich) overnight at room temperature (RT) and transferred to 30% sucrose in PBS for overnight incubation at 4 °C, prior to embedding in OCT compound for cryosectioning. Slides were processed for in-situ hybridization using the RNAscope Multiplex Fluorescent Kit, according to manufacturer instructions using probes Mm-Ppip5k1and Mm-Ip6k2-C2 (Advanced Cell Diagnostics, Newark, CA). Upon completion of the RNAscope assay, slides were incubated in a blocking buffer containing 5% FBS, 1% BSA, and 0.2% Triton X-100 in PBS for 1 hr at RT for immunohistochemistry. Slides were then incubated with biotinylated PNA (1:250; Vector Labs, Burlingame, CA) in blocking buffer overnight at 4 °C, followed by a 0.5 hr incubation with Alexa Fluor 488 streptavidin (1:250, Invitrogen, Carlsbad, CA) in blocking buffer at RT. Fluorescence microscopy images were obtained on a Keyence BZ-X810 fluorescent microscope (Keyence, Itasca, IL).

Sander C.L., Luu J., Kim K., Furkert D., Jang K., Reichenwallner J., Kang M., Lee H.J., Eger B.T., Choe H.W., Fiedler D., Ernst O.P., Kim Y.J., Palczewski K, & Kiser P.D. (2021). Structural evidence for visual arrestin priming via complexation of phosphoinositols. Structure (London, England : 1993), 30(2), 263-277.e5.

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Photoreceptor Quantification in Mouse Retina

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Mice were euthanized in a CO₂ chamber prior to enucleation. Eyes were fixed in Hartman's Fixative Solution (Sigma-Aldrich) overnight at room temperature (RT) and embedded in paraffin. Paraffin sections were stained with hematoxylin and eosin (H&E). Spider plots were generated by manual counting of photoreceptor nuclei in the outer nuclear layer (ONL) at several fixed locations from the optic nerve head, visualized through H&E staining and expressed as the number of nuclei per column.

Kolesnikov A.V., Luu J., Jin H., Palczewski K, & Kefalov V.J. (2022). Deletion of Protein Phosphatase 2A Accelerates Retinal Degeneration in GRK1- and Arr1-Deficient Mice. Investigative Ophthalmology & Visual Science, 63(8), 18.

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