Exosomes were lysed using lysis buffer. Protein lysates of exosomes (20 μg) were run on 4% – 20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules CA, USA) and transferred to Nitrocellulose membrane. The blots were incubated separately either with rabbit polyclonal anti-human CD63 antibody (Santa Cruz Biotechnology Inc. Santa Cruz CA, USA) at a dilution of 1:500 or rabbit polyclonal anti-human Grp-75 (mortalin) antibody (Cat# ab53098, Abcam, Cambridge MA, USA) at a dilution of 1:1000, and incubated at 4°C for overnight followed by washing with TBS buffer. The blots were incubated with secondary antibody, either horseradish peroxidase (HRP)-conjugated goat anti-mouse (Cat# 31460) from ThermoFisher Scientific (Rockford IL, USA) at a dilution of 1:10,000 (5% dry Milk containing TBS buffer) for 1 hour at room temperature. The blots were washed with TBST at room temperature for 1.5 hours (washing/30 min, 3×), and then were treated with the 1:1 ECL buffer A and B (ThermoFisher Scientific Inc.) according to the user manual, developed on image instrument and finally observed using ChemiDoc MP Imaging System Image Lab software (Bio-Rad, USA).
Chemidoc mp imaging system image lab software
Manufactured by Bio-Rad
Sourced in United States
The ChemiDoc MP Imaging System is a versatile imaging platform designed for capturing and analyzing images of gels and blots. The Image Lab software, included with the system, provides a user-friendly interface for image capture, analysis, and data management.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated goat anti mouse, by Thermo Fisher Scientific (2 mentions)
Mini protein tgx gel, by Bio-Rad (2 mentions)
Anti human cd63 antibody, by Santa Cruz Biotechnology (2 mentions)
Anti human grp 75 mortalin antibody, by Abcam (2 mentions)
Ecl buffer a and b, by Thermo Fisher Scientific (2 mentions)
2 protocols using chemidoc mp imaging system image lab software
1
Exosomal Protein Expression Analysis
2
Exosomal Protein Expression Analysis
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Exosomes were lysed using lysis buffer. Protein lysates of exosomes (20 μg) were run on 4% – 20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules CA, USA) and transferred to Nitrocellulose membrane. The blots were incubated separately either with rabbit polyclonal anti-human CD63 antibody (Santa Cruz Biotechnology Inc. Santa Cruz CA, USA) at a dilution of 1:500 or rabbit polyclonal anti-human Grp-75 (mortalin) antibody (Cat# ab53098, Abcam, Cambridge MA, USA) at a dilution of 1:1000, and incubated at 4°C for overnight followed by washing with TBS buffer. The blots were incubated with secondary antibody, either horseradish peroxidase (HRP)-conjugated goat anti-mouse (Cat# 31460) from ThermoFisher Scientific (Rockford IL, USA) at a dilution of 1:10,000 (5% dry Milk containing TBS buffer) for 1 hour at room temperature. The blots were washed with TBST at room temperature for 1.5 hours (washing/30 min, 3×), and then were treated with the 1:1 ECL buffer A and B (ThermoFisher Scientific Inc.) according to the user manual, developed on image instrument and finally observed using ChemiDoc MP Imaging System Image Lab software (Bio-Rad, USA).
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