Sevencompact s210 bio

The acidogenicity of S. mutans biofilm was evaluated using the procedures outlined in our recent study [27 (link)]. Measurement of the pH of the biofilm growth medium was performed by using a microelectrode InLab^® Micro Pro ISM^® connected to a bench-top pH meter, a SevenCompact^™ S210-Bio (Mettler-Toledo GmbH, Greifensee, Switzerland). The microelectrode was calibrated with standard pH buffers (pH 4.01 and 7.00).

The acidification of S. mutans biofilm was determined on the basis of a previously described protocol [13 (link)]. The pH of the biofilm growth medium was measured with a microelectrode InLab^® Micro Pro ISM^® connected to a bench-top pH meter, a SevenCompact^™ S210-Bio (Mettler-Toledo GmbH, Greifensee, Switzerland), and was calibrated using standard pH buffers (pH 4.01 and 7.00).

S. mutans biofilm formation and treatments were performed using the same procedures described above. After 24 h of incubation, the biofilm growth medium (THB) was collected from the wells of all the plates and transferred to 1.5-mL microcentrifuge tubes. The pH of the S. mutans biofilm growth medium collected in the tubes was measured with a microelectrode InLab^® Micro Pro ISM^® connected to a bench-top pH meter SevenCompact^™ S210-Bio (Mettler-Toledo GmbH, Greifensee, Switzerland) at room temperature. The microelectrode was calibrated using standard pH buffers (pH 4.01 and 7.00) prior to and following each measurement.