Shim pack gist hp c18 column

The qualitative and quantitative analyses of HGA were determined on a UPLC-MS/MS 8045 system (Shimadzu, Kyoto, Japan), which equipped with a triple quadrupole mass spectrometer using positive ionization mode and coupled with a UPLC equipped with a Shimadzu Shim-pack GIST-HP C18 column (2.1× 100 mm, 3 μm). Mobile phase A consisted of 0.1% formic acid and 10 mmoL/L ammonium acetate in HPLC-grade water, mobile phase B composed of HPLCgrade methanol. The ow rate was 0.3 mL/min and the elution gradient was programmed as follows: 0 min, 22% B; 1 min, 22% B; 3 min, 25% B; 6 min, 30% B; 8 min, 60% B; 13 min, 80% B; and nally, the initial conditions were held for 3 min to re-equilibrate the column. In addition, the instrument parameters were set as follows: sheath gas ow was 3 L/min, aux gas ow was 10 L/min and heated gas ow was 10 L/min. The temperature of ion transfer tube, DL and heating block were 300, 250 and 400 °C, respectively. Multiple-reaction monitoring (MRM) was used to obtain the quantitative and qualitative data. The quantitation ion of HGA was based on the transition m/z 142.10 → 74.05, with regard to the conformation ion, was on the grounds of the transition m/z 142.10 → 96.05. The collision energies (CE) of HGA were11.0 V.