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12c glucose

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12C-glucose is a stable isotope of glucose used in various analytical and research applications. It is a form of glucose where the carbon-12 isotope is present, as opposed to the more common carbon-13 isotope. This product is utilized in scientific studies and experiments that require the use of a specific carbon isotope of glucose.

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Lab products found in correlation

Benzo h quinoline, by Merck Group (1 mentions) 1 7 phenanthroline, by Merck Group (1 mentions) 4 7 phenanthroline, by Merck Group (1 mentions) Phenanthrene, by Merck Group (1 mentions) 13c glucose, by Cambridge Isotopes (1 mentions) Glucose free dmem, by Thermo Fisher Scientific (1 mentions) U 13c glucose, by Cambridge Isotopes (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)

3 protocols using 12c glucose

1

Synthesis and Characterization of Organic Compounds

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Phenanthrene (Phen), 1,7-phenanthroline (1,7-Phen), 4,7-phenanthroline (4,7-Phen), 12C-glucose and benzo[h]quinoline (B[h]Q) were purchased from Sigma-Aldrich Company Ltd, UK.
Anyanwu I.N, & Semple K.T. (2016). Assessment of the effects of phenanthrene and its nitrogen heterocyclic analogues on microbial activity in soil. SpringerPlus, 5, 279.
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2

Metabolite Extraction and 13C-Glucose Tracing

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Metabolite extraction from medium samples was done by adding 1 mL of conditioned medium to 4 mL of cold (−80°C) 100% methanol, then clarified by centrifugation. Intracellular metabolite fractions were prepared from cells that were lysed with cold (−80°C) 80% methanol, then clarified by centrifugation. Metabolite pellets from intracellular fractions were normalized to the protein content of a parallel sample, and all samples were lyophilized via speed vac. Dried metabolite pellets from cells or media were re-suspended in 35 μL 50:50 MeOH: H2O mixture for metabolomics analysis.
13C-Glucose tracing was performed using glucose free DMEM (Gibco) supplemented with 10% dialyzed FBS (Gibco) and either 25mM 12C glucose (Sigma) or uniformly labeled 13C-glucose (Cambridge Isotopes). BMDM cultures were polarized with appropriate stimuli for 24 hours in normal media, then the media was replaced with 13C or 12C glucose labeling media and incubated overnight. Samples were then harvested and prepared as above.
Halbrook C.J., Pontious C., Kovalenko I., Lapienyte L., Dreyer S., Lee H.J., Thurston G., Zhang Y., Lazarus J., Sajjakulnukit P., Hong H.S., Kremer D.M., Nelson B.S., Kemp S., Zhang L., Chang D., Biankin A., Shi J., Frankel T.L., Crawford H.C., Morton J.P., di Magliano M.P, & Lyssiotis C.A. (2019). Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer. Cell metabolism, 29(6), 1390-1399.e6.
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3

Murine Liver Cancer Cell Culture under Normoxia and Hypoxia

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Primary murine liver cancer cells derived from a Myc- and Akt-driven tumor model (MycOE; AktMyr; Tp53−/−) 15 (link) were a gift from Daniel Dauch (University of Tübingen). Cells were cultured in glucose-free DMEM (Sigma Aldrich) supplemented with 1 mM acetate, 2 mM glutamine and 10% dialyzed FCS after addition of 25 mM of either 12C-glucose (Sigma) or U-13C-glucose (Cambridge Isotopes) in a 37°C incubator with 5% CO2 either under normoxia (20% O2) or hypoxia (0.5% O2) for 72 hours. The time point of 72 hours was chosen to make sure that isotopic steady state for palmitate had been reached. Hypoxic conditions (0.5% O2) were induced in a hypoxia workstation (H35, Don Whitley). The cells cultured under hypoxic conditions were modified to express Green Fluorescent Protein (GFP) as a marker. For co-plating experiments, the normoxic and hypoxic cells were detached using trypsin, mixed using 10,000 cells of each condition, plated on the same glass slide and allowed to attach for 3 hours before fixation.
Buglakova E., Ekelöf M., Schwaiger-Haber M., Schlicker L., Molenaar M.R., Mohammed S., Stuart L., Eisenbarth A., Hilsenstein V., Patti G.J., Schulze A., Snaebjornsson M.T, & Alexandrov T. (2024). 13C-SpaceM: Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer. bioRxiv.
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