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Anti ccr5 apc clone 2d7

Manufactured by BD

Anti CCR5 APC (clone 2D7) is a fluorescently-labeled antibody that binds to the CCR5 chemokine receptor on the surface of cells. The clone 2D7 is a monoclonal antibody conjugated to the APC (Allophycocyanin) fluorescent dye. This product can be used for the identification and enumeration of CCR5-expressing cells in flow cytometry applications.

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2 protocols using anti ccr5 apc clone 2d7

1

Flow Cytometry Immunophenotyping Panel

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The following antibodies were used for flow cytometry: anti Integrin β7 PE (clone FIB504), anti CD45RO APC and anti CD45RO BV421 (clone UCHL1), anti CD62L PE (clone DREG-56), anti CD69 PE (clone FN50), anti CCR5 APC (clone 2D7) and anti Ki-67 FITC (clone MOPC-21) (BD Pharmingen, San Diego, CA). For intracellular Ki-67 staining, BD Perm Wash Kit and company protocol were utilized (BD Pharmingen, San Diego, CA). The anti-α4β7 mAb heterodimer (clone Act-1) was gifted by Dr. Aftab Ansari. The Synagis antibody was gifted from Dr. Barton Haynes and used as a control in several experiments where designated. Data were collected on a FACSCanto II (BD Biosciences, San Diego, CA) and were analyzed using FlowJo and GraphPad Prism software.
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2

Flow Cytometry Immunophenotyping Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry: anti Integrin β7 PE (clone FIB504), anti CD45RO APC and anti CD45RO BV421 (clone UCHL1), anti CD62L PE (clone DREG-56), anti CD69 PE (clone FN50), anti CCR5 APC (clone 2D7) and anti Ki-67 FITC (clone MOPC-21) (BD Pharmingen, San Diego, CA). For intracellular Ki-67 staining, BD Perm Wash Kit and company protocol were utilized (BD Pharmingen, San Diego, CA). The anti-α4β7 mAb heterodimer (clone Act-1) was gifted by Dr. Aftab Ansari. The Synagis antibody was gifted from Dr. Barton Haynes and used as a control in several experiments where designated. Data were collected on a FACSCanto II (BD Biosciences, San Diego, CA) and were analyzed using FlowJo and GraphPad Prism software.
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