The assay kits for catalase, chitinase, lactate dehydrogenase, superoxide dismutase, glutathione, oxidized glutathione, H2O2, reducing sugar, malondialdehyde, and trehalose were purchased from Beijing Solarbio Science & Technology Co., Ltd. The assay methods were conducted according to the instructions of the assay kits.
Malondialdehyde
Manufactured by Solarbio
Sourced in United States, China
Malondialdehyde is a laboratory reagent used in the detection and measurement of lipid peroxidation. It is a naturally occurring compound that serves as a biomarker for oxidative stress and cellular damage. Malondialdehyde can be quantified using colorimetric or fluorometric assays to assess the level of oxidative stress in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Superoxide dismutase, by Solarbio (3 mentions)
Glutathione (gsh), by Solarbio (2 mentions)
Catalase, by Solarbio (2 mentions)
Trehalose, by Solarbio (2 mentions)
Chitinase, by Solarbio (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Bca kit, by Beyotime (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Erastin, by Targetmol (1 mentions)
Micro reduced glutathione gsh assay kit, by Solarbio (1 mentions)
Acsl4, by Santa Cruz Biotechnology (1 mentions)
Nissl stain solution, by Solarbio (1 mentions)
Tublin, by Cell Signaling Technology (1 mentions)
Micro malondialdehyde mda assay kit, by Solarbio (1 mentions)
Iron assay kit, by Nanjing Jiancheng (1 mentions)
Phosphate buffered saline (pbs), by Sartorius (1 mentions)
Bca protein quantitation kit, by Thermo Fisher Scientific (1 mentions)
Kaempferol, by Solarbio (1 mentions)
Cck 8, by Solarbio (1 mentions)
4 protocols using malondialdehyde
1
Antioxidant Enzyme Activity Assay
2
Ferroptosis Regulation in Nervous System
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The chemical and reagents used were Nissl stain solution (cresyl violet, G1430, Solarbio), malondialdehyde (MDA) assay kit; glutathione (GSH) assay kit, dimethyl sulfoxide (DMSO), BCA kits (Beyotime Biotechnology, Beijing), rabbit antibodies against antiglutathione peroxidase 4 (GPX4, ABclonal, USA, A13309), anticystine/glutamate antiporter (SLC7A11, ab175186, Abcom, USA), ACSL4 (sc-271800, Santa Cruz Biotechnology Inc., USA), Nrf2 (ABclonal, USA), GAPDH (1 : 1000, Cell Signaling Technologies, United States, #5174), tublin (1 : 1000, Cell Signaling Technologies, United States). Micro malondialdehyde (MDA) Assay Kit (BC0025, Solarbio Science & Technology Co., Ltd., Beijing.), and Micro Reduced Glutathione (GSH) Assay Kit (BC1175, Solarbio Science & Technology Co., Ltd., Beijing). Iron Assay Kit was obtained from Nanjing Jiancheng Co. (A039-2, China). Reactive Oxygen Species Assay Kit was obtained from Beyotime Biotechnology (S0033, Beijing). Fetal bovine serum (FBS) was purchased from GIBCO Inc. (Logan, Utah, USA). Erastin (T1765, TargetMol, USA) and RSL3 (HY-100218A, MedChemExpress, USA) were also used.
3
Evaluating Antioxidant Potential of Kaempferol
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Kaempferol, AAPH, superoxide dismutase (SOD), malondialdehyde (MDA), and cytotoxicity assay (CCK-8) kits were obtained from Solarbio (Beijing, China). 3,5-dimethylphenol was obtained from Life Technologies (Carlsbad, CA, United States). Phosphate-buffered saline (PBS) was procured from Biological Industries (Ridgefield, CT, United States). Penicillin–streptomycin solution was obtained from ScienCell (San Diego, CA, United States). Dulbecco’s modified Eagle’s medium (DMEM) and trypticase were purchased from HyClone (Logan, UT, United States). Fetal bovine serum (FBS) was procured from Gibco (Waltham, MA, United States). A BCA Protein Quantitation Kit was obtained from Thermo Fisher Scientific (Waltham, MA, United States).
4
Antioxidant Enzyme Activity Assay
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The assay kits for catalase, chitinase, lactate dehydrogenase, superoxide dismutase, glutathione, oxidized glutathione, H2O2, reducing sugar, malondialdehyde, and trehalose were purchased from Beijing Solarbio Science & Technology Co., Ltd. The assay methods were conducted according to the instructions of the assay kits.
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