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Anti tbx5 ab

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Anti-Tbx5 Ab is a primary antibody that specifically recognizes the Tbx5 protein. Tbx5 is a transcription factor involved in heart and limb development. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and study the Tbx5 protein.

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2 protocols using anti tbx5 ab

1

Immunohistochemistry for Neural Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against Pax6 (1:10), N-cadherin (6B3, 1:10), β-catenin (PY-489, 1:20) and Laminin (3H11, 1:10) were obtained from Developmental Studies Hybridoma Bank (Iowa City, Iowa, USA). Anti-Pax2 Ab (1:100) was obtained from Covance (Princeton, NJ, USA). Anti-Tbx5 Ab (1:100) and Anti-Gli1 (H-300, 1:50) were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Anti-Mitf Ab (1:500) was obtained from Abcam (Cambridge, MA, USA). Anti-Chx-10 (Vsx2, 1:250) Ab was purchased from ExAlpha (Shirley, MA, USA). Fluorescein/Oregon Green Ab (1:100) was purchased from Molecular Probes (Grand Island, NY, USA). The production of Abs against C3 (targets amino acids 741–761) and C3aR as well as the blocking peptides are described in Haynes et al. (2013) (link). Preimmune IgG was isolated from the corresponding rabbit serum using Protein A affinity purification. All secondary Abs were purchased from Molecular Probes (Grand Island NY, USA) and used at 1:100 dilutions.
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2

Immunohistochemistry for Neural Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against Pax6 (1:10), N-cadherin (6B3, 1:10), β-catenin (PY-489, 1:20) and Laminin (3H11, 1:10) were obtained from Developmental Studies Hybridoma Bank (Iowa City, Iowa, USA). Anti-Pax2 Ab (1:100) was obtained from Covance (Princeton, NJ, USA). Anti-Tbx5 Ab (1:100) and Anti-Gli1 (H-300, 1:50) were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Anti-Mitf Ab (1:500) was obtained from Abcam (Cambridge, MA, USA). Anti-Chx-10 (Vsx2, 1:250) Ab was purchased from ExAlpha (Shirley, MA, USA). Fluorescein/Oregon Green Ab (1:100) was purchased from Molecular Probes (Grand Island, NY, USA). The production of Abs against C3 (targets amino acids 741–761) and C3aR as well as the blocking peptides are described in Haynes et al. (2013) (link). Preimmune IgG was isolated from the corresponding rabbit serum using Protein A affinity purification. All secondary Abs were purchased from Molecular Probes (Grand Island NY, USA) and used at 1:100 dilutions.
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