Immpress excel staining kit

Manufactured by Vector Laboratories

Sourced in United States

The ImmPRESS Excel Staining Kit is a laboratory product designed for immunohistochemical staining. It provides a sensitive detection system for visualizing target antigens in tissue samples.

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Lab products found in correlation

Vectastain elite abc hrp kit, by Vector Laboratories (1 mentions) Nanozoomer slide scanning system, by Hamamatsu Photonics (1 mentions) Bz x710 all in one fluorescence microscope, by Keyence (1 mentions)

Spelling variants of this product

ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (4 citations) ImmPRESS kits (3 citations) ImmPRESS Excel Amplified Polymer Staining kit (2 citations) ImmPRESS™ Excel Amplified HRP (peroxidase) Polymer Staining Kit (2 citations)

5 protocols using immpress excel staining kit

Immunohistochemical Analysis of NICD in Paraffin-Embedded Tissues

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For immunohistochemical stainings of paraffin-embedded sections, tissues were fixed in 4%
PFA at 4°C overnight, embedded in paraffin according to standard laboratory protocols, sectioned at 5 μm, and mounted on glass slides. Antigen retrieval was done by boiling in a steam pressure pot in 10 mM sodium citrate buffer pH 6.0 for 10 minutes. For immunohistochemical staining of NICD, sections were incubated with BLOXALL (ImmPRESS Excel Staining Kit; Vector laboratories, cat. no. MP-7601) to inactivate endogenous peroxidases for 10 minutes, washed in PBS, permeabilized with PBST (0.2% Triton X-100 in PBS) for 10 minutes, blocked with 4% FCS in PBST for at least 1 hour, and incubated with anti-NICD antibody (diluted in 2% FCS in PBST) at 4°C overnight, washed with PBS, followed by usage of the ImmPRESS Excel Staining Kit (Vector laboratories, cat. no. MP-7601).
Sections were then dehydrated and mounted with Pertex.

Chen J., Javed A., Kaiser L., Nava M.M., Zhao D., Brandt D.T., Fernández-Baldovinos J., Zhou L., Höß C., Sawmynaden K., Oleksy A., Matthews D.J., Weinstein L.S., Gröne H., Niessen C.M., Offermanns S., Wickström S.A., & Worzfeld T. (2020). Mechanochemical control of epidermal stem cell divisions by B-plexins.

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Comprehensive Immunohistochemical Profiling of Cancer Tissue Microarrays

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Immunohistochemical staining was performed on FFPE slides using the VECTASTAIN Elite ABC HRP Kit (Vector, PK-6100), ImmPRESS Excel Staining Kit (Vector, MP-7602), and ImmPRESS HRP anti-rat IgG, mouse adsorbed (peroxidase) polymer detection kit (Vector, MP-7444). Slides were imaged on a NanoZoomer Slide Scanning System (Hamamatsu), and the area fraction for each protein with respect to tumor tissue was calculated utilizing ImageJ (NIH) [23 ]. Integrin β3 levels on tumor cells for cancer microarray slides were analyzed blindly, and the tissues were categorized into β3- and β3+ groups. Microarray slides were purchased from Biomax.us: Lung cancer (LC10011a, 50 cases/100 cores, grades 2–3; LC121c, 120 cases/120 cores, grades 1–3; HLugC120PT01, 60 cases/60 cores, grades 1–3), Prostate cancer (PR483c, 48 cases/48 cores, grades 1–3), Colon carcinoma (CO1006, 50 cases/100 cores, grades 1–3), Kidney clear cell cancer (Hkid-CRC060CS-01, 30 cases/60 cores, grades 1–4; BC07001, 40 cases/80 cores, grades 1–3), Multiple organs (MC1801, 180 cases/180 cores, containing 26 cases of colon, pancreas, lung, breast and prostate cancer, grades 1–3) and Brain glioblastoma (GL805, 40 cases/80 cores, grades 3–4).

Wettersten H.I., Weis S.M., Pathria P., Von Schalscha T., Minami T., Varner J.A, & Cheresh D.A. (2019). Arming tumor-associated macrophages to reverse epithelial cancer progression. Cancer research, 79(19), 5048-5059.

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Immunohistochemistry of Muscle Tissue

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Following dissection, the muscle was immediately frozen in liquid nitrogen-cooled isopentane and mounted in Tissue Tech freezing medium (Jung) cooled by dry ice/ethanol. Immunohistochemistry was performed on 10 μm cryosections that were dried for 30 min before the application of block wash buffer (PBS with 5% foetal calf serum (v/v), 0.05% Triton X-100). Antibodies were diluted in wash buffer 30 min before use. Details of primary and secondary antibodies are given in Supplementary file 1. F4/80 was detected using the Vector Laboratories ImmPRESS Excel Staining Kit. Morphometric analysis of fibre size was performed as previously described (Matsakas et al., 2012a (link)).

Omairi S., Matsakas A., Degens H., Kretz O., Hansson K.A., Solbrå A.V., Bruusgaard J.C., Joch B., Sartori R., Giallourou N., Mitchell R., Collins-Hooper H., Foster K., Pasternack A., Ritvos O., Sandri M., Narkar V., Swann J.R., Huber T.B, & Patel K. (2016). Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres. eLife, 5, e16940.

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Quantification of Tumor Fibrosis and Angiogenesis

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Tumor tissues fixed in PFA were paraffin-embedded, sectioned, and stained with hematoxylin and eosin (H–E) followed by fibrosis staining with Masson’s Trichrome as described [24 (link)]. Photomicrographs of Masson’s trichrome-stained tumors were analyzed using ImageJ v.1.54i processing software to quantify the proportion of blue stain collagen tissue as a percentage of the total cross-sectional area. Immunohistochemistry of serial sections was performed with antibodies to collagen I (abcam34710, 1:100), alpha smooth muscle actin (abcam15734 1:100), or CD31 (abcam28364, 1:50). Staining was visualized using an ImmPRESS Excel Staining Kit (MP-7601, Vector Labs, Newark, CA, USA) with a one-minute DAB incubation and documented with a BZ-X710 All-in-one fluorescence microscope (Keyence, Elwood Park, NJ, USA) under bright field conditions. Quantitative IHC image analysis was also performed using ImageJ software, and at least 10 fields per slide were analyzed.

Abraham T., Armold M., McGovern C., Harms J.F., Darok M.C., Gigliotti C., Adair B., Gray J.L., Kelly D.F., Adair J.H, & Matters G.L. (2024). CCK Receptor Inhibition Reduces Pancreatic Tumor Fibrosis and Promotes Nanoparticle Delivery. Biomedicines, 12(5), 1024.

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Immunohistochemistry for Tissue Analysis

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Immunohistochemistry was performed on 10 μm cryosections as described previously [33] .
Antibodies against myosin heavy chain isoforms, cluster of differentiation 31 (CD31), 3nitrotyrosine (3NT), laminin, 8-hydroxy-2'-deoxyguanosine (8OH-dG) and E06 were used. Details of primary and secondary antibodies are given in Supplementary Table 1. F4.80 was detected using the Vector Laboratories ImmPRESS Excel Staining Kit.

Sfyri P.P., Yuldasheva N.Y., Tzimou A., Giallourou N., Crispi V., Aburima A., Beltran-Alvarez P., Patel K., Mougios V., Swann J.R., Kearney M.T, & Matsakas A. (2018). Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity. Free radical biology & medicine, 129.

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