Odyssey imaging instrument

Manufactured by LI COR

Sourced in United States

The Odyssey imaging instrument is a highly sensitive and versatile platform for detecting and quantifying fluorescent signals in a variety of applications, including Western blotting, multiplex assays, and protein-protein interactions. The Odyssey system utilizes near-infrared fluorescence technology to provide accurate and reliable results, with the ability to simultaneously detect multiple targets in a single experiment.

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Lab products found in correlation

Irdye 800cw, by LI COR (3 mentions) Pbs blocking buffer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Odyssey infrared imaging instrument Odyssey color infrared laser scan-imaging instrument Odyssey Infrared Laser Scan-imaging Instrument Odyssey NIR fluorescence imaging instrument Odyssey instrument

5 protocols using odyssey imaging instrument

Protein Separation and Detection

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Total protein lysates were mixed with sample buffer and heated at 95 °C for 5 min. Equal amounts of protein were subjected to SDS/PAGE and transferred to nitrocellulose. The membranes were blocked in protein‐free PBS blocking buffer (Thermo Scientific) for 1 h at room temperature. Primary and secondary Ab incubations were performed in protein‐free blocking buffer/0.05% Tween‐20 for 1 h at room temperature. For all membranes, protein signals were detected using an Odyssey imaging instrument and analyzed using instrument software (Li‐Cor Biosciences, Lincoln, NE, USA).

Katara G.K., Kulshrestha A., Schneiderman S., Riehl V., Ibrahim S, & Beaman K.D. (2019). Interleukin‐22 promotes development of malignant lesions in a mouse model of spontaneous breast cancer. Molecular Oncology, 14(1), 211-224.

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Quantitative Western Blot Analysis

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Equal amounts of total protein lysates were mixed with sample buffer, heated at 95°C for 5 min and loaded to SDS-PAGE and transferred to nitrocellulose. The membranes were blocked in protein-free PBS blocking buffer (Thermo Scientific, Rockford, IL) for 1 h at room temperature. Primary and secondary antibodies were incubated in protein-free blocking buffer/0.05% Tween- 20 for 1 h at room temperature. Protein signals were detected using an Odyssey imaging instrument and analyzed using instrument software (Li-Cor Biosciences). The intensities of the immunoreactive bands were quantified by densitometric analysis using ImageJ software.

Ibrahim S.A., Katara G.K., Kulshrestha A., Jaiswal M.K., Amin M.A, & Beaman K.D. (2015). Breast cancer associated a2 isoform vacuolar ATPase immunomodulates neutrophils: potential role in tumor progression. Oncotarget, 6(32), 33033-33045.

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Quantitative Slot Blot for E. coli HCPs

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A quantitative slot blot assay was previously developed for determining the amount of E. coli HCPs in recombinantly expressed proteins [24 (link)] and was modified by using IRDye 800CW (LI-COR Biosciences) as the secondary antibody and measured with an Odyssey imaging instrument (LI-COR Biosciences) at 800 nm.

Burkhardt M., Reiter K., Nguyen V., Suzuki M., Herrera R., Duffy P.E., Shimp R J.r., MacDonald N.J., Olano L.R, & Narum D.L. (2018). Assessment of the impact of manufacturing changes on the physicochemical properties of the recombinant vaccine carrier ExoProtein A. Vaccine, 37(38), 5762-5769.

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Quantitative Slot Blot Assay for E. coli HCPs

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A quantitative slot blot immunoassay was previously developed for determining the amount of E. coli HCPs in recombinantly expressed proteins [22 (link)] and was modified by using IRDye 800CW (LI-COR Biotechnology, Lincoln NE, USA) as the secondary antibody. Briefly, E. coli with no insert was fermented, the cells were isolated and disrupted using a microfluidizer. The soluble fraction from the cell lysate was used for generating a polyclonal antibody reagent. A standard curve was performed by 1:2 dilutions of the E. coli proteins and used to calculate the HCP level in the test sample. Fluorescence was measured at 800 nm with an Odyssey imaging instrument (LI-COR Biotechnology, Lincoln NE, USA).

Signorello L.B., Harlow B.L., Chekos A.K, & Repke J.T. (2000). Midline episiotomy and anal incontinence: retrospective cohort study. BMJ : British Medical Journal, 320(7227), 86-90.

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Quantitative Slot Blot Assay for E. coli HCPs

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Signorello L.B., Harlow B.L., Chekos A.K, & Repke J.T. (2000). Midline episiotomy and anal incontinence: retrospective cohort study. BMJ : British Medical Journal, 320(7227), 86-90.

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