Dulbecco s modified eagle s medium dmem

Manufactured by Bioidea

Dulbecco's modified eagle's medium (DMEM) is a cell culture medium commonly used for the maintenance and growth of various cell types. It provides a balanced salt solution and a source of energy, amino acids, and vitamins to support cell proliferation and viability. DMEM is a versatile medium that can be used for a wide range of cell culture applications.

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4 protocols using dulbecco s modified eagle s medium dmem

Cadmium Chloride Cytotoxicity Assay

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Cadmium chloride (CdCl₂), low-melting-point agarose (LMA), ethidium bromide, Tris-HCL, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA. Osthole is a gift from Dr. Sajjadi. Dulbecco's modified eagle's medium (DMEM) was achieved by Bioidea company, Iran, and Biosera, France supplied fetal bovine serum (FBS) and penicillin/streptomycin. H₂O₂, NaCl, KH₂PO₄, and KCL were procured from Merck Company, Germany. All reagents were of analytical grade.

Vahidifar E., Sajjadi S.E, & Etebari M. (2022). Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay. Research in Pharmaceutical Sciences, 17(6), 657-664.

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Nanomedicine for Colorectal Cancer

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All reagents and materials utilized in the present work were handled under RNase-free conditions. 5-FU, chitosan (75 kDa, 75–85% degree of deacetylation), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 4‘,6-diamidino-2-phenylindole (DAPI), polyvinylidene fluoride (PVDF), coumarin-6 (C6; ≥99%), sodium hyaluronate (HA), lipid components including cholesterol (van Noort et al., 2014 (link)), hydrogenated soybean phosphatidylcholine (HSPC) and 1,2-dipalmitoyl-sn-glycerol-3-phosphoglycerol (DPPG) were obtained from Sigma-Aldrich. A human SW480 cell line and a mouse colorectal cancer cell line CT-26 was purchased from the National Cell Bank (Pasteur Institute, Iran). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin (pen-strep 100X solution) were purchased from the Bioidea company, Iran. Phosphate buffer solution (PBS) was obtained from DNAbiotech company, Iran. All RNA sequences were prepared from Metabion (Germany). All antibodies were bought from Santa Cruz Biotechnologies.

Shahidi M., Abazari O., Dayati P., Haghiralsadat B.F., Oroojalian F, & Tofighi D. (2022). Targeted delivery of 5-fluorouracil, miR-532-3p, and si-KRAS to the colorectal tumor using layer-by-layer liposomes. Frontiers in Bioengineering and Biotechnology, 10, 1013541.

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Cytotoxicity and Antioxidant Evaluation

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PCA was obtained from Cayman Chemical Co. (USA; Cat No. 14916-25) as a synthetic material with ≥ 98% purity. DOX was prepared from EBEWE Pharma GmbH Nfg KG Co. (Austria; Cat No. 118529), and ATO from Bahar Sabz Talaie Pharmacy (Iran; Global Trade Item No. 06262749061436). Fetal bovine serum (FBS) was purchased from Biosera Co. (France). Dulbecco's modified eagle's medium (DMEM) was obtained from BioIdea Co. (Iran). MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay kit was purchased from Alfa Aesar GmbH & Co KG (Germany). The assay kits for the determination of total oxidant capacity and ferric-reducing antioxidant power (FRAP) were prepared by Hakiman Shargh Research Co. (Iran). The lactate dehydrogenase (LDH) release assay kit was from Kiazist (Iran). BIOFACT^TM total RNA Prep kit was purchased from BioFact Ltd. (Korea), the cDNA synthesis kit from Yekta Tajhiz Azma Co. (Iran), and the Quantitect SYBR Green master mix kit from Qiagen (Germany). All other chemicals were from Merck Co., Germany.

Shafiee F., Safaeian L, & Gorbani F. (2023). Protective effects of protocatechuic acid against doxorubicin- and arsenic trioxide-induced toxicity in cardiomyocytes. Research in Pharmaceutical Sciences, 18(2), 149-158.

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Evaluation of Cell Viability and Insulin Assay

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RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Trypsin-EDTA and MTT (2, 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) were purchased from Bio-Idea Company (Tehran. Iran). BSA was purchased from Solarbio (Life Sciences). Penicillin–streptomycin was purchased from Gibco Life Technologies (Paisley, UK). Glucose oxidase was purchased from Pars Azmon Company (Tehran, Iran). The insulin radioimmunoassay kit was purchased from Mercodia. The protein assay reagent was obtained from Bio-Rad (Copenhagen, Denmark). The chemicals including sulfuric acid, ethanol, Na₂So₄, KOH, all were purchased from Merk (Germany).

Safarzad M., Marjani A., Saghaeian Jazi M., Qujeq D., Mir S.M., Marjani M, & Nezhadebrahimi Kaldehi A. (2020). Effect of Rubus anatolicus Leaf Extract on Glucose Metabolism in HepG2, CRI-D2 and C2C12 Cell Lines. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 1109-1116.

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