The protein lysates from CT26 and HCT116 cells were separated by SDS‐PAGE and then transferred to a PVDF membrane (Millipore). The membrane was incubated with the primary Abs against anti‐HIF1α (1:1000, Ab179483; Abcam), anti‐TRIB3 (1:1000, Ab137526; Abcam), anti‐β‐catenin (1:1000, Ab32572; Abcam), anti‐Wnt3A (1:1000, Ab219412; Abcam), and anti‐β‐actin (1:1000, Ab8226; Abcam), followed by incubation with an HRP‐conjugated secondary Ab (1:1000; Abcam).
Hrp conjugated secondary ab
Manufactured by Abcam
Sourced in United States
HRP-conjugated secondary Ab is a lab equipment product that functions as a detection reagent. It is a type of antibody that is conjugated with horseradish peroxidase (HRP), an enzyme commonly used in various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (1 mentions)
Ab219412, by Abcam (1 mentions)
Ab137526, by Abcam (1 mentions)
Ab179483, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
Ab32572, by Abcam (1 mentions)
Bcl 2, by Abcam (1 mentions)
Ecl detection kit, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Solarbio (1 mentions)
Ecl detection kit, by Cell Signaling Technology (1 mentions)
3 protocols using hrp conjugated secondary ab
1
Western Blot Analysis of Cellular Proteins
2
Western Blotting of Integrin and Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell pellets were lysed by RIPA Lysis Buffer (Solarbio), and the protein suspension was boiled with loading buffer for 10 minutes, separated by SDS‐PAGE, and transferred onto PVDF membranes. Then the samples were blocked with 5% BSA and incubated overnight at 4°C with primary Abs against: integrin β8 (1:300), p‐YBX1 (1:500), YBX1 (1:500), NF‐κB (c‐rel) (1:500), BCL2 (1:500), and actin (1:500; all Abcam). Samples were incubated with HRP‐conjugated secondary Ab (1:1000, Abcam) for 1 hour at room temperature and then visualized by the ECL Detection Kit (Thermo Fisher Scientific).
3
Integrin α2β1 expression analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were solubilized with an equal volume of loading buffer (125 mmol/L Tris‐HCl, pH 6.8, 4% SDS, 20% glycerol, 0.05% bromophenol blue, and 5% β‐mercaptoethanol) and boiled for 10 minutes. Samples were separated by SDS‐PAGE then transferred to PVDF membranes and detecting by immunoblotting with primary Abs against integrin α2β1 (1:1000; Abcam) and actin (1:1000; Abcam) at 4°C overnight. After that, HRP‐conjugated secondary Ab (1:1000; Abcam) was incubated for 1 hour at room temperature and visualized by the ECL Detection Kit (Cell Signaling Technology, Danvers, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!