Whole-cell extracts were quantified by the bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse IgG HRP conjugated (Jackson). Flow cytometry was performed on FACSCantoII (BD Bioscences) and analyzed by FlowJo Software.
Anti cd155 pvr pe
Anti-CD155/PVR-PE is a fluorescent-labeled antibody that binds to the CD155/PVR (poliovirus receptor) antigen. CD155/PVR is a cell surface protein that functions as a receptor for the poliovirus and is also involved in cell-cell adhesion.
Lab products found in correlation
2 protocols using anti cd155 pvr pe
Comprehensive Antibody Characterization for Western Blot and Flow Cytometry
Whole-cell extracts were quantified by the bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse IgG HRP conjugated (Jackson). Flow cytometry was performed on FACSCantoII (BD Bioscences) and analyzed by FlowJo Software.
Immune Profiling of NB Tumor Cells
Flow cytometry was performed on FACSCantoII and analysed by FACSDiva Software (BD Biosciences).
ROS production was evaluated in drug-treated NB cell lines by using CellROX Deep Red Reagent (C10422, Invitrogen) and measured by flow cytometry.
Whole-cell extracts were quantified by a bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse and HRP-conjugated rabbit anti-goat IgG (Jackson). The following antibodies for Western blotting were used: anti-p53 (FL-393) and anti-actin (I-19), purchased by Santa Cruz Biotechnology.
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