The experimental mice were anesthetized, and the skin around eye was pulled away to allow the eye to bulge out of the socket. Then a sterile capillary tube was penetrated into the corner of the eye socket underneath the eyeball to collect blood from orbital sinus into an Eppendorf tube. The samples were centrifuged (4°C, 3000 rpm, 15 min), then the supernatants were collected into Eppendorf tubes and stored at -80°C until use. The concentrations of leptin, insulin and triglycerides were detected by using the ELISA kits (leptin: KE10048, Proteintech; Insulin: ab277390, Abcam; Triglyceride: MAK266, Sigma-Aldrich.), according to the manufacturer’s instructions.
Ke10048
Manufactured by Proteintech
Sourced in United States
The KE10048 is a laboratory centrifuge designed for general-purpose use. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 4,000 g. The unit is equipped with a fixed-angle rotor that can accommodate up to 8 sample tubes. The centrifuge is suitable for a variety of applications requiring sample separation and concentration.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ke10048
1
Orbital Blood Collection for Metabolic Markers
2
Serum Lipid and Adipokine Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum was analysed for free fatty acid (FFA), triglyceride (TG), total cholestero (TC), HDL‐C, and LDL‐C using the Bayer 1650 blood chemistry analyser (Bayer AG, Leverkusen, Germany) and for adiponectin and leptin using ELISA kits (Proteintech KE10044 and KE10048, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!