Recombinant murine interferon (IFN)‐γ was purchased from PeproTech (Rocky Hill, NJ, USA). The following monoclonal antibodies (mAb) and polyclonal antibodies (pAb) were used. PE‐conjugated anti‐mouse PD‐L1 mAb (MIH1; BD Biosciences, San Jose, CA, USA) was used for flow cytometry. Goat anti‐mouse PD‐L1 pAb (AF1019; R&D Systems, Minneapolis, MN, USA) was used for western blotting. Rabbit anti‐mouse CD4 mAb (Abcam, Cambridge, MA, USA), rabbit anti‐mouse CD8α pAb (Abcam), rat anti‐mouse CD49b mAb (BD Pharmingen, Piscataway, NJ, USA), rabbit anti‐mouse Foxp3 pAb (BioLegend, San Diego, CA, USA), rat anti‐mouse F4/80 mAb (AbD Serotec, Oxford, UK), and rabbit anti‐mouse PD‐1 mAb (Abcam) were used for immunohistochemistry. Rabbit anti‐CD11c pAb (Bioss, Woburn, MA, USA), rabbit anti‐CD206 pAb (Abcam), rat anti‐F4/80 mAb (BMA Biomedicals, Rheinstrasse, Switzerland), rabbit anti‐CXCL9 pAb (Bioss) and rabbit anti‐CXCL10 pAb (Bioss) were used for double‐color immunofluorescence analyses.
Recombinant murine interferon ifn γ
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant murine interferon (IFN)‐γ is a laboratory reagent produced using recombinant DNA technology. IFN‐γ is a cytokine that plays a crucial role in immune response and regulation.
Automatically generated - may contain errors
2 protocols using recombinant murine interferon ifn γ
1
Immunophenotyping of Murine Immune Cells
2
Podocyte Culture and Differentiation
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MPC5 cells were donated by Prof. Weijing Liu (Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, China). Podocytes were cultured at 33 °C in medium that consisted of Roswell Park Memorial Institute (RPMI) 1640 medium (11879020/11875093, Gibco, NY, USA) supplemented with 10% foetal bovine serum (10099-141, Gibco), 100 μg/mL streptomycin, 100 U/mL penicillin G (V900929, Sigma, MO, USA), and 100 U/mL recombinant murine interferon (IFN)-γ (315-05-20, PeproTech, NJ, USA) to facilitate proliferation. Then, the podocytes were cultured at 37 °C for 10–14 days in RPMI 1640 medium without IFN-γ to facilitate cell differentiation. The podocytes were used for the in vitro experiment when the confluence was approximately 80%. The differentiated cells were stimulated for 48 h with normal glucose (NG, 5.5 mM), HG (HG, 30 mM), and BSTL (H + B, 30 mM glucose + BSTL drug-containing serum). All experimental results were verified in at least three independent podocyte cultures.
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