Wdr82

Manufactured by Abcam

Sourced in United States

Wdr82 is a protein that is involved in the regulation of gene expression. It is a component of the histone methyltransferase complex and plays a role in the epigenetic modification of chromatin.

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Lab products found in correlation

2 7 dichlorofluorescin diacetate dcfda, by Merck Group (1 mentions) Histone h3, by Merck Group (1 mentions) Rna polymerase 2, by Abcam (1 mentions) Rbbp5, by Cell Signaling Technology (1 mentions) H3k4me3, by Abcam (1 mentions) Ptbp1, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) H3k27ac, by Abcam (1 mentions) Cd206, by R&D Systems (1 mentions) Grp94, by Santa Cruz Biotechnology (1 mentions) 0.22 μm polyvinylidene fluoride membrane, by Merck Group (1 mentions) Tsg101, by Santa Cruz Biotechnology (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using wdr82

Antibody Panel for Protein Analysis

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The following antibodies were used in this study: FLAG (F1804; 1:2,000 for Western blot [WB]) and β-actin (A1978; 1:10,000 for WB) from Sigma-Aldrich; histone H3 (ab1791; 1:10,000 for WB), RNA polymerase II (phospho S5; ab5408; 1:200 for ChIP), H3K4me3 (ab8580; 1:200 for ChIP and Cut&Tag), and H3K27ac (ab6002; 1:200 for Cut&Tag) from Abcam; Wdr82 (99715; 1:1,000 for WB; 1:100 for Cut&Tag), DDX5 (9877T; 1:1,000 for WB), and RBBP5 (13171S; 1:200 for ChIP) from Cell Signaling Technology; PCBP2 (NBP2-19715; 1:1,000 for WB) from Novusbio; PTBP1 (32-4800; 1:1,000 for WB) from Invitrogen; α-globin (14537-1-AP; 1:2,000 for WB) from Proteintech; EKLF (OM184222; 1:1,000 for WB) and β-globin (OM256195; 1:1,000 for WB) from OmnimAbs; and ASH2L (A11278; 1:200 for ChIP) from Abclonal. Anti-FLAG M2 affinity gel (A2220), 3× FLAG peptide (F4799), doxycycline (D9891), PHZ (P26252) and 2′,7′-dichlorofluorescin diacetate (DCF-DA; 35845) were purchased from Sigma-Aldrich. Guinea pig anti-rabbit IgG (heavy & light chain) antibody (ABIN101961; 1:100 for Cut&Tag) was from Antibodies Online. Tetramethylrhodamine methyl ester (TMRE; I34361) and 2-NDBG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose; N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03), murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

Yang S., Sun G., Wu P., Chen C., Kuang Y., Liu L., Zheng Z., He Y., Gu Q., Lu T., Zhu C., Wang F., Gou F., Yang Z., Zhao X., Yuan S., Yang L., Lu S., Li Y., Lv X., Dong F., Ma Y., Yu J., Ng L.G., Shi L., Liu J., Shi L., Cheng T, & Cheng H. (2022). WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturation. The Journal of Experimental Medicine, 219(4), e20211688.

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Protein Extraction and Western Blot Analysis

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The total protein was extracted from tissues, cells and exosomes using modified RIPA buffer and sonication, and the protein concentration was detected by BCA method. Total protein extract (20 µg) or exosomal protein (10 µg) was separated using a 10% or 15% polyacrylamide gel and transferred to a 0.22-μm polyvinylidene fluoride membrane (Merck Millipore, USA). The membrane was blocked with 5% skim milk for 1 h, and incubated with the primary antibodies WDR82 (1: 100), β-actin (1: 1000, Abcam), CD206 (1: 1000, R&D Systems, Minneapolis, MN, USA), ARG1 (1: 1000, Proteintech, Chicago, USA), CD81 (1:1000), Alix (1: 1000), TSG101 (1: 1000), GRP94(1:500) (Santa Cruz Biotechnology, CA, USA) overnight at 4 ℃, and the corresponding secondary antibody for 1 h. The membrane was reacted with enhanced chemiluminescence solution for 5 min and detected in the exposure apparatus.

Song L., Luan B., Xu Q., Shi R, & Wang X. (2022). microRNA-155-3p delivered by M2 macrophages-derived exosomes enhances the progression of medulloblastoma through regulation of WDR82. Journal of Translational Medicine, 20, 13.

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