Clinical specimens were processed by standard microbiological methods.[7 ] Proteus species were provisionally diagnosed based on nonlactose-fermenting colonies on MacConkey agar media (Himedia, Vadhani Industrial Estate, LBS Marg, Mumbai, India) with (or without) swarming on blood agar media (Himedia, Vadhani Industrial Estate, LBS Marg, Mumbai, India). Identification of Proteus was done by biochemical tests to find whether they were positive for phenylalanine deaminase production, H2S gas production, citrate utilization, and urease production. Proteus vulgaris produces indole which differentiates it from indole-negative Proteus mirabilis and Proteus penneri. Maltose fermentation and lack of ornithine decarboxylase differentiated P. penneri from P. mirabilis.[7 ]
Blood agar media
Manufactured by HiMedia
Sourced in India
Blood agar media is a laboratory culture medium used for the growth and identification of microorganisms. It contains nutrients essential for microbial growth, and is supplemented with defibrinated blood, typically from sheep or horse. This medium supports the growth of a wide range of bacteria and fungi.
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Lab products found in correlation
Bhi broth, by HiMedia (2 mentions)
King s b kb media, by HiMedia (2 mentions)
Mueller hinton media, by HiMedia (2 mentions)
Macconkey agar media, by HiMedia (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Middlebrook 7h9 medium, by BD (1 mentions)
Acetonitrile and methanol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Petri plates, by HiMedia (1 mentions)
Plga 50 50, by Durect (1 mentions)
L wenstein jensen lj medium, by HiMedia (1 mentions)
Dulbecco s modified eagle s medium dmem, by HiMedia (1 mentions)
5 protocols using blood agar media
1
Identification of Proteus Species
2
Mosquito Larvae Microbiome Cultivation
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Homogenates of the mosquitoes (3 larvae per sample) were suspended in 1 ml of sterile aqueous Tween-20 (0.03%), and the suspensions were then diluted 50-fold. Next, 100 µl aliquots were inoculated onto the surface of modified Sabouraud agar (10 g peptone, 40 g D-glucose anhydrous, 20 g agar, 1 g yeast extract) supplemented with an antibiotic cocktail (acetyltrimethyl ammonium bromide 0.35 g/L; cycloheximide 0.05 g/L; tetracycline 0.05 g/L; streptomycin 0.6 g/L; PanReacAppliChem, Germany) for the inhibition of bacteria and saprotrophic fungi. The Petri dishes were maintained at 28 °C in the dark. The colonies were then counted after 7 days.
For the estimation of cultivated bacterial CFU counts, homogenates of larvae (3 larvae per sample) were suspended in 1 ml of 0.1 M phosphate buffer. Then, the suspension was diluted to 10−2, 10−3 and 10−4. Aliquots of 100 µl of the larval dilutions were inoculated onto the surface of blood agar media (HiMedia, Mumbai, India). The Petri dishes were maintained at 28 °C. The colonies were counted after 48 h. Three samples of each treatment were used in the analysis.
For the estimation of cultivated bacterial CFU counts, homogenates of larvae (3 larvae per sample) were suspended in 1 ml of 0.1 M phosphate buffer. Then, the suspension was diluted to 10−2, 10−3 and 10−4. Aliquots of 100 µl of the larval dilutions were inoculated onto the surface of blood agar media (HiMedia, Mumbai, India). The Petri dishes were maintained at 28 °C. The colonies were counted after 48 h. Three samples of each treatment were used in the analysis.
3
Development of PLGA Nanoparticles for Tuberculosis
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PLGA 50:50 (MW: 1,00,000–1,20,000 Da) was procured from Durect Corporation (Birmingham, AL, USA), RIF from Duchefa-Genetix Biotech, acetonitrile and methanol from Merck Millipore (Billerica, MA, USA) and INH, benzaldehyde, ethyl alcohol and trifluoroacetic acid (TFA) from Loba Chemie (Mumbai, India). The rest of the reagents and chemicals used were of high-performance liquid chromatography (HPLC) grade. Polyvinyl alcohol (PVA) (87%–89% hydrolyzed, MW: 1,20,000 Da) was procured from Thomas Baker (Mumbai, India). Middlebrook 7H9 medium, antimicrobial mixture MGIT™ PANTA™ and MGIT™ growth supplement oleic acid-albumin-dextrose-catalase (OADC) were procured from Becton Dickinson (Franklin Lakes, NJ, USA). Löwenstein–Jensen (LJ) medium, blood agar media, Petri plates and disposable loops were procured from HiMedia (Mumbai, India). The cell culture requirements, Ham’s F-12 medium, Dulbecco’s Modified Eagle’s Medium (DMEM; HiMedia), fetal bovine serum (FBS) and Coumarin 6 were procured from Sigma-Aldrich (St. Louis, MO, USA).
4
Preparation of Microbial Culture Media
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King's B (KB) media (HiMedia, India), Mueller Hinton media (HiMedia, INdia), Blood agar media (HiMedia, India) and BHI broth (HiMedia, INdia) were prepared by adding agar into the distilled water. Hot plate was used for the proper mixing of media and autoclaved at 121°C for 15-20 minutes at 15 lbs.
5
Preparation of Microbial Growth Media
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King's B (KB) media (HiMedia), Mueller Hinton media (HiMedia), Blood agar media (HiMedia) and BHI broth (HiMedia) were prepared by adding agar into the distilled water. Hot plate was used for the proper mixing of media and autoclaved at 121° C for 15-20 minutes at 15 lbs.
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