The reprogramming procedure was conducted as previously described (Park et al., 2008b (link)). Detroit 551 cells were seeded at 100,000 cells/well of a six-well plate 1 day prior to infection. A retrovirus cocktail containing OSKM was added to each well at MOI 5. On day 5 post-infection, the cells were trypsinized and transferred to 10-cm culture dishes containing MEFs. Prior to sorting, the cells were detached using accutase, washed, and incubated in 20% FBS in 1× PBS with the following antibodies according to manufacturer’s recommended dilutions: anti-human CD13 (BD catalog number 555394), anti-human/mouse SSEA4 (R&D catalog number FAB1435A), anti-human TRA160 (BD catalog number 560193). Sorting was conducted using a BD FACSAria cell sorter. Then the cells were pelleted and quickly frozen in liquid nitrogen or sorted directly in RLT + 2-mercaptoethanol lysis buffer (QIAGEN).
Anti human cd13
Manufactured by BD
Sourced in Italy
Anti-human CD13 is a laboratory reagent used for the identification and enumeration of CD13-positive cells in biological samples. CD13 is a cell surface antigen expressed on various cell types, including myeloid cells, endothelial cells, and fibroblasts. This reagent can be utilized in flow cytometry and other immunoassay applications to detect and quantify CD13-expressing cells.
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Lab products found in correlation
Facsaria cell sorter, by BD (1 mentions)
Anti human hla dr, by BD (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Fcs express 4 flow research edition software, by De Novo Software (1 mentions)
Anti human cd73, by BD (1 mentions)
Anti human cd90, by Merck Group (1 mentions)
Isotype matched irrelevant monoclonal antibodies, by BD (1 mentions)
2 protocols using anti human cd13
1
Reprogramming of Human Somatic Cells
2
Immunophenotyping of Mesenchymal Stem Cells
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For immunophenotype studies, dual-color immunofluorescence was performed using the following panel of phycoerythrin (PE)-conjugated or fluorescein isothiocyanate (FITC)-conjugated monoclonal antibodies: anti-human CD14, anti-human CD13, anti-human HLA-DR, anti-human CD34, and anti-human CD73 (BD Biosciences, Buccinasco, Italy); and anti-human CD105, anti-human CD44, anti-human CD45, anti-human CD90, and anti-human CD29 (Chemicon, Temecula, CA, USA). Negative controls were isotype-matched irrelevant monoclonal antibodies (BD Biosciences). MSCs (5 × 105) were incubated in the dark for 15 minutes at 4 °C in PBS–1 % bovine serum albumin (BSA). Cells were rinsed in PBS and analyzed using a BD Accuri C6 flow cytometer (BD Biosciences). A minimum of 10,000 events was collected in list mode on FCS Express 4 Flow Research Edition Software (De Novo software, Glendale, CA, USA).
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