Myocyte supplements mix

The human metastatic BC cells, e.g., MDA-MB-231 (purchased from ATCC), were maintained in the Dulbecco's Modified Eagle's Medium (Gibco-BRL) supplemented with 10% fetal bovine serum (FBS) (Euroclone), 2 mM L-glutamine (Euroclone), 100 U/ml penicillin, and 100 μg/ml streptomycin (Euroclone), at 37°C, 5% CO₂. The human BC cell line BT549 (purchased from ATCC) was grown in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin, and 0.023 U/ml insulin at 37°C, 5% CO₂. The human cardiomyocytes (HCMs, purchased by PromoCell) cells were maintained in the Myocyte Growth Medium (PromoCell) plus Myocyte supplements mix (PromoCell), in addition to 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 37°C, 5% CO₂. The rat cardiomyocytes cell line H9C2 (purchased by PromoCell) was maintained in DMEM-F12, supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. All the cell lines used in the study were routinely checked for eventual mycoplasma contamination, before being used.