Fibronectin from human plasma (F0895) and l -histidinol dihydrochloride (H6647) were obtained from Sigma-Aldrich. Hygromycin (#ant-hg) was obtained from InvivoGen. For Western blot, anti-Pyk2 (3292) was obtained from Cell Signaling Technology; anti-FAK (610088) and anti CrkI/II (610036) were obtained from BD Transduction Laboratories; anti-CrkL (sc-319) was obtained from Santa Cruz Biotechnology; anti-GFP (11814460001) was obtained from Roche Life Science; anti–β-actin (clone AC-15) (A5441) was obtained from Sigma-Aldrich; anti–HSC-70 (1427) was obtained from Abcam. Secondary antibodies (goat anti-mouse 680LT and goat anti-rabbit 800CW) were obtained from LI-COR Biosciences. For immunofluorescence, antivinculin (V9131) was obtained from Sigma-Aldrich; rhodamine-labeled phalloidin and Alexa Fluor–conjugated secondary antibodies were obtained from Molecular Probes.
L histidinol dihydrochloride h6647
Manufactured by Merck Group
L-histidinol dihydrochloride (H6647) is a chemical compound used in various research and laboratory applications. It is a salt of the amino acid histidinol. The product is available in powder form for use in experiments and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti vinculin v9131, by Merck Group (1 mentions)
Rhodamine labeled phalloidin, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit 800cw, by LI COR (1 mentions)
Goat anti mouse 680lt, by LI COR (1 mentions)
Anti β actin clone ac 15 a5441, by Merck Group (1 mentions)
Anti crkl sc 319, by Santa Cruz Biotechnology (1 mentions)
Anti gfp 11814460001, by Roche (1 mentions)
Anti fak 610088, by BD (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Thapsigargin t9033, by Merck Group (1 mentions)
Triptolide t 3652, by Merck Group (1 mentions)
Hoechst solution 33 258, by Merck Group (1 mentions)
α amanitin, by Merck Group (1 mentions)
Fugene hd transfection reagent, by Promega (1 mentions)
2 protocols using l histidinol dihydrochloride h6647
1
Fibronectin and Histidinol Immunoblotting Protocol
2
HaloTag Fluorescent Labeling Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HaloTag R110Direct Ligand G3221 and HaloTag TMRDirect Ligand G2991 were purchased from Promega. Hoechst solution (33,258; used at 1:1000 dilution), L-histidinol dihydrochloride (H6647; used at 2 mM), all-trans-Retinoic acid (R-2625; used at 10 μm), thapsigargin (T-9033; used at 300 nM), and triptolide (T-3652, used at 500 μM) were all from Sigma. α-Amanitin (used at 10 μM) was purchased from Sigma (A2263) or EMD/Millipore (129741). FuGENE HD transfection reagent was obtained from Promega.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!