Porcine angiotensinogen

Manufactured by Merck Group

Sourced in United States

Porcine angiotensinogen is a laboratory product used in research and scientific experiments. It is derived from porcine (pig) sources and functions as a precursor protein in the renin-angiotensin system. This product is intended for use in controlled laboratory settings by trained professionals.

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Lab products found in correlation

Neurotensin, by Merck Group (2 mentions) Bradykinin, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Human angiotensin 2, by Merck Group (2 mentions) Substance p, by Merck Group (2 mentions) Kemptide, by Merck Group (2 mentions) Human angiotensin 1, by Merck Group (2 mentions) Scp0021, by Merck Group (1 mentions) Td 96208, by Inotiv (1 mentions) 8 hydroxyquinoline, by Merck Group (1 mentions) Fibrinopeptide a, by Merck Group (1 mentions) Ang 1 enzyme immunoassay eia kit, by Bachem (1 mentions) Melittin, by Merck Group (1 mentions)

Close spelling variants of this product

Angiotensinogen 1–14 renin substrate porcine Angiotensinogen

4 protocols using porcine angiotensinogen

Plasma Renin Activity Measurement in Olfr558 Mice

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Plasma renin activity was measured in 3-month-old Olfr558 WT and KO mice with a modified angiotensin I measurement kit (S-1188, Peninsula Laboratories). Plasma was collected from male and female Olfr558 WT and KO mice treated with 0.49% NaCl diet (catalog no. TD.96208 modified with orange color, Envigo). From males, we also collected plasma from mice given Teklad diet (Teklad 2018SX, 18% protein). Plasma was diluted 15-fold and then incubated with excess porcine angiotensinogen (SCP0021, Sigma-Aldrich) for 20 min at 37°C in a buffer containing 50 mM sodium acetate (pH 6.5), 10 mM 4-(2-aminoethyl) benzenesulfony fluoride hydrochloride (Sigma-Aldrich), 10 mM EDTA (pH 8.0), 1 μM porcine angiotensinogen, and 10 mM 8-hydroxyquinoline (Sigma-Aldrich). After incubation, the sample was analyzed according to the provided protocol. Plasma renin activity was assayed by competitive binding of angiotensin I antibody.

Xu J., Choi R., Gupta K., Warren H.R., Santhanam L, & Pluznick J.L. (2024). An evolutionarily conserved olfactory receptor is required for sex differences in blood pressure. Science Advances, 10(12), eadk1487.

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Preparation of Peptide Standards for Mass Spectrometry

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1 mg/mL stock solutions of each peptide (human angiotensin I, human angiotensin II, bradykinin, fibrinopeptide A, kemptide, neurotensin, porcine angiotensinogen, and substance P, all purchased from Sigma-Aldrich) were prepared in 0.1% formic acid (FA, Sigma-Aldrich, St. Louis, MO) in 10% acetonitrile (ACN, Fisher Scientific, Pittsburgh, PA) and deionized water. (Barnstead Nanopure Infinity System, Dubuque, IA). A peptide mixture containing 10 μM of each peptide was prepared from the stock solution in the same solvent mixture. Prior to MS analysis the peptide mixture solutions with final concentrations of 1 μM and 100 nM for each peptide were prepared by further dilution.

Cox J.T., Marginean I., Smith R.D, & Tang K. (2014). On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces. Journal of the American Society for Mass Spectrometry, 26(1), 55-62.

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Plasma Renin Concentration Measurement

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PRC measurement was performed on the 7th day of normal salt (NS), low salt (LS), or high salt (HS) diets. For hemorrhagic shock experiments, PRC was measured at 0, 1, 3, 9, 15 and 20 hours post hemorrhagic shock as previously reported [16 (link)]. The mice were lightly anesthetized with 1.5% isoflurane. Blood samples (~25 μL/sample) were collected from the retro-orbital plexus and centrifuged for 10 minutes at 1500 × g. Plasma samples (~10 μL) were obtained and incubated with excess porcine angiotensinogen (0.4 μmol/L; Sigma, St. Louis, MO, USA) for 20 min. The amount of angiotensin (Ang) I generated after incubation was used to calculate the PRC with the Ang I enzyme immunoassay (EIA) kit (Bachem, San Carlos, CA, USA). PRC was expressed as the amount of Ang I generated per hour per milliliter of plasma.

Liu C., Wang X., Parris C., Pang Q., Naeem M.U, & Wang L. (2023). Macula Densa Nitric Oxide Synthase-1 Controls Renin Release and Renin-Dependent Blood Pressure Changes. Discovery medicine, 35(177), 525-532.

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Peptide Quantification by Mass Spectrometry

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The ESI solvent consisted of 0.1% formic acid (FA, Sigma-Aldrich, St. Louis, MO) in 10% acetonitrile (ACN, Fisher Scientific, Pittsburgh, PA) and deionized water (Barnstead Nanopure Infinity System, Dubuque, IA). Stock solutions of 9 peptides (human angiotensin I, human angiotensin II, bradykinin, fibrinopeptide, kemptide, melittin, neurotensin, porcine angiotensinogen, and substance P, all purchased from Sigma-Aldrich) were prepared in the ESI solvent. Aliquots from the stock solutions were mixed and diluted to a final concentration of 1 μM for each peptide.

Cox J.T., Marginean I., Kelly R.T., Smith R.D, & Tang K. (2014). Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures. Journal of the American Society for Mass Spectrometry, 25(12), 2028-2037.

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