Ubiquitin clone fk2

The following commercial antibodies were used in this study: monoclonal antibodies to p62 (BD Biosciences), LC3 (MBL), GFP (Abcam), ubiquitin (clone FK2; Enzo); polyclonal antibodies to Tom1/Tom1L2 (Abcam), GIPC (Santa Cruz), actin (Sigma), Salmonella (Abcam), and GFP (Life Technologies). Biotin-conjugated anti-ubiquitin antibody, clone P4D1, was purchased from eBiosciences. Rabbit polyclonal antibodies against myosin VI, TAX1BP1, NDP52, and optineurin were produced as previously described [11 (link), 23 (link), 24 (link)].
Human recombinant linear tetra-ubiquitin (Ub4), K63-linked polyubiquitin chains (2–7), and K48-linked polyubiquitin chains (2–7) were purchased from BostonBiochem (R&D Systems Europe Ltd.).

Primary antibodies BAG6 (Proteintech, 26417‐1‐AP), Calnexin (Abcam, ab22595), C9orf72 (GeneTex, GTX632041), C9orf72 (Proteintech, 25757‐1‐AP), GET4 (Proteintech, 27768‐1‐AP), GFP (Roche, 11814460001), HA (Cell Signaling, 3724S), NRF2 (Abcam, ab62352), PSMC4 (Bethyl Laboratories, A303‐850A), SGTA (Proteintech, 11019‐2‐AP), SMCR8 (Abcam, ab202283), α‐tubulin (Abcam, ab7291), Ubiquitin clone FK2 (Enzo Life Sciences, BML‐PW8810), Ubiquitin K11 clone 2A3/2E6 (Genentech), Ubiquitin K48 clone Apu2 (Millipore, 05‐1307), Ubiquitin K63 clone Apu3‐A8 (Genentech), Ubiquitin K11/K48‐bispecific (Genentech), UBL4A (Proteintech, 14253‐1‐AP) and UBR5 (Cell Signaling, 65344) were used for immunoblotting at a dilution of 1:1,000 in 5% milk in Tris‐buffered saline with Tween20 (TBS‐T). C9orf72 (Proteintech, 22637‐1‐AP) antibody was used for immunofluorescence staining at a dilution of 1:50 in 0.1% BSA in PBS. For secondary antibodies in immunoblotting anti‐mouse‐HRP (Promega, W402B), anti‐rabbit‐HRP (Promega, W401B), and anti‐human‐HRP (Promega, W403B) were applied at a dilution of 1:10,000. For immunofluorescence, the secondary antibody goat anti‐rabbit AlexaFluor™594 (Life Technologies, A11012) was used at a dilution of 1:1,000.