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3 protocols using disodium phosphate dodecahydrate

1

Multifunctional Nanomaterial Synthesis

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1×TBE buffer: tris(hydroxymethyl)aminomethane (Tris, 89 mM), chloroauric acid (HAuCl4), disodium ethylenediaminetetraacetic (EDTA, 2 mM), polyethylene glycol (PEG, Mw = 5,000), bis(p-sulfonateophenyl)phenylphosphine dihydrate dipotassium (BSPP), sodium dihydrogen phosphate (NaH2PO4.2H2O), disodium phosphate dodecahydrate (Na2HPO4.12H2O), fluorescein isothiocyanate (FITC), dithiothretol (DTT) and sodium borohydride (NaBH4) were obtained from Sigma-Aldrich (USA). Sodium dodecylsulfate (SDS), sodium citrate (Na3C6H5O7), sodium chloride (NaCl) were analytical grade reagent from Guangdong Guanghua Chemical Reagent Co. (China). DNA oligonucleotides were synthesized by Sangon Biotech. Co. (China). Fetal calf serum (FCS), 4′,6-diamidino-2-phenylindole (DAPI), renal tubular epithelial cells and 0.05% trypsin from American Type Culture Collection. N-acetylation chitosan (NACS) was prepared according to the previous method in our group43 (link). All the reagents were used as received without further purification. Milli-Q water (>18.0 MΩ cm) was used to prepare all aqueous solutions.
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2

Quantum Dots for Neurotransmitter Detection

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Oleic acid-capped quantum dots (QDs-OA) with alloyed (CdSexS1-x) core and ZnS shell (CdSeS/ZnS), nanocrystal diameter = 6.0 nm, λem = 630 nm, 1 mg/mL in toluene were purchased from Cytodiagnostics Inc. (Burlington, Canada). Glutathione in reduced form (GSH) was from Acros Organics (Geel, Belgium). Tetramethylammonium hydroxide pentahydrate (TMAH) was from Alfa Aesar (Kandel, Germany). Chloroform, anhydrous ethanol, and phosphoric acid (85%) were obtained from Chempur (Piekary Śląskie, Poland). Dialysis tubing cellulose membrane (MWCO = 12 kDa), dopamine hydrochloride, L-epinephrine, L-norepinephrine, serotonin hydrochloride, γ-aminobutyric acid (GABA), acetylcholine chloride, sodium phosphate monohydrate, disodium phosphate dodecahydrate, and sodium hydroxide were supplied by Sigma-Merck (Poznań, Poland). Milli-Q water was used for preparation of all aqueous solutions. All chemicals were used as received.
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3

Simulated Hospital Solution Bioaerosols

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The chemicals used to prepare the simulated hospital aqueous solutions (urine, saliva, untreated urban wastewater) have been specified in the supplementary material.
Escherichia coli ATCC25922 provided from Scharlab, Spain, was used as a model of bacteria in this research. A sterile phosphate buffer solution (PBS) was used to collect bioaerosols and it was constituted of sodium chloride, potassium chloride, disodium phosphate dodecahydrate and potassium dihydrogen phosphate received from Sigma Aldrich.
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