The molecular weight, LA/GA molar ratio and PLGA/PEG weight ratio of the copolymer were determined by means of proton nuclear magnetic resonance 1H NMR spectroscopy on a Bruker (Billerica, MA, USA) ADVANCE III HD 700 MHz instrument in deuterated chloroform (CDCl3) solvent at 25 °C (Supplementary Figure S1 ). The number-average molecular weight (Mn) and polydispersity index (Mw/Mn) of the PLGA–PEG–PLGA copolymer were determined via gel permeation chromatography with the multi-angle light scattering method (GPC-MALS) (Supplementary Figure S2 ). The instrumental setup included an Agilent HPLC 1100 Series instrument with a degasser, pump, autosampler, set of two PLgel 5 μm Mixed-C 300 × 7.5 mm columns (Agilent, Santa Clara, CA, USA) thermostated to 25 °C and a UV-VIS diode array detector connected to a DAWN HELEOS II multi-angle laser light scattering detector, a ViscoStar-II differential viscometer and Optilab T-rEX refractive index detectors (Wyatt Technology, Dernbach, Germany). Tetrahydrofuran was used as the mobile phase at a flow rate of 1 mL·min−1.
Advance 3 hd 700 mhz
Manufactured by Bruker
Sourced in United States
The ADVANCE III HD 700 MHz is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced analytical applications. It features a 700 MHz magnet and provides high-resolution NMR spectroscopy capabilities for the characterization of a wide range of chemical and biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Optilab t rex refractive index detector, by Wyatt Technology (1 mentions)
Plgel 5 μm mixed c 300 7.5 mm columns, by Agilent Technologies (1 mentions)
Viscostar 2 differential viscometer, by Wyatt Technology (1 mentions)
Dawn heleos 2 multi angle laser light scattering detector, by Wyatt Technology (1 mentions)
Maxis hd, by Bruker (1 mentions)
1200 series, by Bruker (1 mentions)
1260 infinity hplc system, by Agilent Technologies (1 mentions)
Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions)
Acetonitrile d3, by Cambridge Isotopes (1 mentions)
3 protocols using advance 3 hd 700 mhz
1
Characterization of PLGA-PEG-PLGA Copolymer
2
Platinum Compound Characterization by LCMS and NMR
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LCMS measurements were performed using an HPLC (Agilent technologies 1200 series) equipped with a Gemini-NX 3u C18 110A 50 × 2.0 mm column coupled to an ion trap mass spectrometer (Bruker amaZon SL). High resolution mass spectra were recorded by direct infusion into a Q-TOF mass spectrometer (Bruker maXis HD) using electrospray ionization (ESI) in the positive mode. 1H and 195Pt NMR spectra were recorded using a Bruker Advance-III HD 700 MHz spectrometer. Chemical shifts are reported as values in ppm, for the 1H-NMR relative to residual solvent signal as internal standard and for the 195Pt-NMR relative to the reference compound Na2PtCl6.
3
Synthesis and Characterization of Ruthenium(II) Complexes
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All the chemicals were purchased from commercial sources and used as received without further purification. The complexes Δ/Λ-[Ru(bipy) 2 ( py
were prepared by the method of von Zelewsky. 11 For the studies undertaken in water, ruthenium hexafluorophosphate salts were converted to chloride salts by using Amberlite® IRA-410 chloride form. Aqueous solutions were prepared using a PURELAB® Option-Q water purification system. High-field nuclear magnetic resonance (NMR) spectra including 1 H-NMR, 13 C-NMR, COSY, HSQC, HMBC were collected on a Bruker Advance III HD 700 MHz spectrometer. Chemical shifts for all complexes were reported relative to acetonitrile-d 3 (Cambridge Isotope Laboratories, CD 3 CN, 99.8%) at δ = 1.940 ppm. FT-IR spectra were recorded on a PerkinElmer FT-IR Spectrum Two spectrometer. Chromatography experiments were performed on Agilent 1260 Infinity HPLC system equipped with Raptor C18 column (100 × 3 mm; 2.7 μm particle size) using water/ acetonitrile 1/9 mixture as eluent (0.3 mL min -1 ) and compared to the solvent blank run. Diode Array Detector (1260 DAD VL+) was fixed in the spectral region of interest at 567 nm and at 250 nm to detect any non-photoactive impurities. Highresolution electron spin ionization (ESI) mass spectrometry was collected by National Mass Spectrometry Facility (NMSF) in Swansea.
were prepared by the method of von Zelewsky. 11 For the studies undertaken in water, ruthenium hexafluorophosphate salts were converted to chloride salts by using Amberlite® IRA-410 chloride form. Aqueous solutions were prepared using a PURELAB® Option-Q water purification system. High-field nuclear magnetic resonance (NMR) spectra including 1 H-NMR, 13 C-NMR, COSY, HSQC, HMBC were collected on a Bruker Advance III HD 700 MHz spectrometer. Chemical shifts for all complexes were reported relative to acetonitrile-d 3 (Cambridge Isotope Laboratories, CD 3 CN, 99.8%) at δ = 1.940 ppm. FT-IR spectra were recorded on a PerkinElmer FT-IR Spectrum Two spectrometer. Chromatography experiments were performed on Agilent 1260 Infinity HPLC system equipped with Raptor C18 column (100 × 3 mm; 2.7 μm particle size) using water/ acetonitrile 1/9 mixture as eluent (0.3 mL min -1 ) and compared to the solvent blank run. Diode Array Detector (1260 DAD VL+) was fixed in the spectral region of interest at 567 nm and at 250 nm to detect any non-photoactive impurities. Highresolution electron spin ionization (ESI) mass spectrometry was collected by National Mass Spectrometry Facility (NMSF) in Swansea.
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