Cells were grown on sterile glass slides, fixed with 4% paraformaldehyde for 30 min, blocked with 1% BSA for 30 min, incubated with primary antibodies against CDH1 (E-cadherin, AF0131, 1:500; Affinity Biosciences LTD.), CDH2 (N-cadherin AF4039, 1:200; Affinity Biosciences LTD.), and VIM (Vimentin AF7013, 1:250; Affinity Biosciences LTD.) at 4°C overnight, followed by incubation with Alexa Fluor® 594 conjugated Affinipure Goat Anti-Rabbit IgG (H + L) secondary antibodies (Jackson ImmunoResearch Laboratories, PA) for 1 hour. Nuclei were counterstained with DAPI. Images were captured with Olympus IX81 inverted fluorescence microscope (Olympus, Beijing, China).
Alexa fluor 594 conjugated affinipure goat anti rabbit igg h l secondary antibodies
Manufactured by Jackson ImmunoResearch
Alexa Fluor® 594 conjugated Affinipure Goat Anti-Rabbit IgG (H + L) secondary antibodies are a product designed for use in immunological assays. They are a Goat-derived secondary antibody that is conjugated to the Alexa Fluor® 594 fluorescent dye. This allows for the detection and visualization of rabbit primary antibodies in various applications.
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2 protocols using alexa fluor 594 conjugated affinipure goat anti rabbit igg h l secondary antibodies
1
Cell Adhesion and Cytoskeleton Proteins
2
Immunofluorescence Analysis of EMT Markers
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Cells were grown on sterile glass slides, fixed with 4% paraformaldehyde for 30 min, blocked with 1% BSA for 30 min, incubated with primary antibodies against E-cadherin CDH1 (AF0131, 1:500; Affinity Biosciences Ltd.), N-cadherin (AF4039, 1:200; Affinity Biosciences Ltd.), and Vimentin (AF7013, 1:250; Affinity Biosciences Ltd.) at 4 °C overnight, and then incubated with Alexa Fluor® 594 conjugated Affinipure Goat Anti-Rabbit IgG (H + L) secondary antibodies (Jackson ImmunoResearch Laboratories, PA) for 1 hour. Nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole). Images were captured with Olympus IX81 inverted fluorescence microscope (Olympus, Beijing, China).
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