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Infrared dye 680cw goat

Manufactured by LI COR
Sourced in United States

The Infrared dye 680cw goat is a near-infrared fluorescent dye used in various bioanalytical applications. It can be used for labeling proteins, antibodies, or other biomolecules. The dye exhibits absorption and emission wavelengths in the near-infrared region, making it suitable for detection in biological samples.

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2 protocols using infrared dye 680cw goat

1

Apoptosis Signaling Pathway Profiling

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The following primary antibodies were used: mouse anti-active caspase-8 (Cell Signaling Technology, Beverly, USA), rabbit anti FAS, and anti FADD antibodies (Santa Cruz Biotechnology), murine activating anti-CD95/FAS (50 ng/ml) from upstate signaling, rabbit anti-AIF IgG (Sigma-Aldrich), mouse anti-p53 antibody (Millipore), Rabbit anti-Flag (Thermo/Fisher Scientific), rabbit anti-nur77 IgG (Santa Cruz), mouse anti–β-actin (Abcam). The following secondary antibodies were used: Infrared dye 800cw goat, anti-rabbit antibody, Infrared dye 680cw goat, anti-mouse antibody (Licor), Rhodamine redX anti-rabbit antibody (Life Technologies), cy5 anti-murine antibody (Abcam).
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2

Antibody Validation Protocol

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The following primary antibodies were used: mouse anti-® actin (Abcam, UK). α/β-Tubulin Antibody (Cell signaling), Anti-cyclin B1 (Millipore, Germany), Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Millipore, Germany). The following secondary antibodies were used: Infrared dye 800cw goat, anti-rabbit antibody, Infrared dye 680cw goat, anti-mouse antibody (Licor, USA), Rhodamine redX antirabbit antibody (life technologies), cy5 anti-murine antibody (Abcam, UK).
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