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2 protocols using nci h1793

1

KRAS Mutation Cell Line Characterization

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Authenticated NCI-H358, NCI-H1792, and NCI-H1793 cell lines were purchased from ATCC. NCI-H1793 harbor homozygous wild-type KRAS, both NCI-H358 and NCI-H1792 are heterozygous for KRASG12C; KRAS is not amplified in any of the cell lines [51 (link)]. Cells were grown in RPMI 1640 Medium supplemented with 10% fetal bovine serum (FBS). Cells were cultured at 37°C in a humidified incubator at 95% O2 and 5% CO2 and were routinely passaged at 80% confluency. All cell lines were confirmed negative for mycoplasma.
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2

Culture of Lung Adenocarcinoma Cell Lines

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Human lung adenocarcinoma cell lines A549, NCI-H2228, NCI-H1975, HCC4006, NCI-H23, Calu-3, NCI-H1435 and NCI-H1793 were purchased from ATCC (VA, USA). The cell line A549 was grown in DMEM/F12 medium (Invitrogen, CA, USA) and other cell lines were grown 1640 medium (Invitrogen), with both mediums supplemented with 10% FBS (Invitrogen) and 1% Penicillin/Streptomycin (Wisent Inc., St-Bruno, Canada). All the cell lines were cultured at 37 • C in 5% CO 2 and 95% air.
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