Goat polyclonal anti gfp

Manufactured by Rockland Immunochemicals

Goat polyclonal anti-GFP is a laboratory reagent produced by Rockland Immunochemicals. It is an antibody raised in goats against green fluorescent protein (GFP).

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Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Goat anti chicken alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Alexafluor goat anti mouse 568, by Thermo Fisher Scientific (1 mentions) Nanogold, by Nanoprobes (1 mentions)

Spelling variants of this product

Goat anti-GFP (46 citations) Anti-GFP (27 citations) Polyclonal anti-GFP (2 citations)

3 protocols using goat polyclonal anti gfp

Antibody-based immunostaining protocol

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Antibodies used in this study include goat polyclonal anti-GFP (Rockland Immunochemicals, Inc, #600-101-215), rabbit polyclonal mLANA anti-serum [21 ], mouse polyclonal MHV68 anti-serum [49 (link)], chicken anti-ORF59 IgY (Gallus Immunotech), and mouse monoclonal anti-β-actin (Sigma Aldrich, #A2228). Fluorophore-conjugated secondary antibodies used in this study include AlexaFluor donkey anti-goat 488, AlexaFluor goat anti-mouse 568, and AlexaFluor goat anti-chicken 568 (Life Technologies). For drug treatments, the 17β-estradiol agonist Z-4-hydroxytamoxifen (4-OHT; Alexis Biochemicals; #ALX-550-361-M001) was dissolved at a stock concentration of 2 mM. Inducible-Cre 3T3 fibroblasts plated the previous day were treated with either 0.2 μM 4-OHT to induce nuclear translocation of Cre or ethanol as a vehicle control for 24 h prior to infection.

Salinas E., Gupta A., Sifford J.M., Oldenburg D.G., White D.W, & Forrest J.C. (2018). Conditional mutagenesis in vivo reveals cell type- and infection stage-specific requirements for LANA in chronic MHV68 infection. PLoS Pathogens, 14(1), e1006865.

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Immunohistochemical Profiling of Embryonic Tissues

Cited 1 time

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Fixed embryos were sectioned at 16μm by cryostat. In situ hybridization and immunohistochemistry were performed as described (Tsuchida et al., 1994 (link)). Antibodies against Hox proteins, LIM HD proteins and other proteins were generated or obtained as described (Dasen et al., 2005 (link); Jung et al., 2010 (link); Liu et al., 2001 (link); Tsuchida et al., 1994 (link)). Additional antibodies used were: monoclonal anti-HA 1:10,000 (Covance), goat polyclonal anti-GFP 1:4,000 (Rockland).

Jung H., Mazzoni E.O., Soshnikova N., Hanley O., Venkatesh B., Duboule D, & Dasen J.S. (2014). Evolving Hox Activity Profiles Govern Diversity in Locomotor Systems. Developmental cell, 29(2), 171-187.

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Immunogold Labeling of GFP

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As previously described (44) samples were high-pressure frozen, freeze-substituted and rehydrated (110), followed by indirect immunogold labeling of 100 nm-thick, thawed cryosections (44) . Antibodies used were goat polyclonal anti GFP (1:500; Rockland) and rabbit anti goat Fab' NANOGOLD™ (1:150; Nanoprobes). Transmission electron microscopy was performed on a Philips CM120 (now: Thermo Fisher Scientific).

Schmidt O., Weyer Y., Sprenger S., Widerin M.A., Eising S., Baumann V., Angelova M., Loewith R., Stefan C.J., Hess M.W., Fröhlich F., & Teis D. (2020). Multiple roles for the ESCRT machinery in maintaining plasma membrane homeostasis.

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