Neomycin trisulfate salt hydrate (Sigma-Aldrich), paromomycin sulfate salt (Sigma-Aldrich), ribostamycin sulfate salt (Sigma-Aldrich), neamine hydrochloride (Toronto Research Chemicals), puromycin dihydrochloride (Sigma-Aldrich) were used at the purity stated by the commercial suppliers (≥ 97%). Chemoenzymatic preparation of acetylated forms of neomycin is described below. 6′-deoxy-paromomycin pentaacetate salt was synthesized by the Vasella group (ETH Zurich) as described48 and was a kind gift from David Crich (Wayne State University). All smFRET experiments were performed in Tris-polymix buffer (pH 7.5) containing 50 mM Tris acetate, pH 7.5, 5 mM Mg(OAc)2, 100 mM KCl, 5 mM NH4OAc, 0.5 mM CaCl2, 0.1 mM EDTA, 5 mM putrescine and 1 mM spermidine.
Neamine hydrochloride
Manufactured by LGC
Neamine hydrochloride is a chemical compound used in laboratory settings. It is a salt formed by the reaction of neamine and hydrochloric acid. The core function of this product is to serve as a reagent or intermediate in various chemical reactions and analyses conducted in a laboratory environment.
Automatically generated - may contain errors
2 protocols using neamine hydrochloride
1
Chemoenzymatic synthesis of acetylated aminoglycosides
2
Chemoenzymatic Synthesis of Acetylated Aminoglycosides
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Neomycin trisulfate salt hydrate (Sigma-Aldrich), paromomycin sulfate salt (Sigma-Aldrich), ribostamycin sulfate salt (Sigma-Aldrich), neamine hydrochloride (Toronto Research Chemicals) and puromycin dihydrochloride (Sigma-Aldrich) were used at the purity stated by the commercial suppliers (≥97%). Chemoenzymatic preparation of acetylated forms of neomycin is described below. 6′-deoxy-paromomycin pentaacetate salt was synthesized by the Vasella group (ETH Zurich) as described48 and was a kind gift from David Crich (Wayne State University). All smFRET experiments were performed in Tris-polymix buffer (pH 7.5) containing 50 mM Tris acetate, pH 7.5, 5 mM Mg(OAc)2, 100 mM KCl, 5 mM NH4OAc, 0.5 mM CaCl2, 0.1 mM EDTA, 5 mM putrescine and 1 mM spermidine.
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