Avance 3 400 mhz spectrophotometer

Melting points were detected by the Tomas–Hoover capillary melting apparatus without any correction. All solvents, chemicals, and reagents were obtained from Aldrich Chemical Company (Milwaukee, WI), and El Nasr pharmaceutical chemical companies, Cairo, Egypt. Infrared (IR) spectra were obtained using films on KBr diks on a Schimadzu FT-IR 8400S spectrophotometer and values were presented as cm⁻¹. The purity of the synthesized compounds and the reaction progress was monitored using precoated thin layer chromatography (TLC) silica gel plates 60F254 with a thickness of 0.25 supplied from MERCK, Darmstadt, Germany. The UV lamp was used to monitor the reaction process. ¹³C NMR and ¹H NMR spectra were measured on a Bruker Avance III 400 MHz spectrophotometer (faculty of pharmacy, Benisuef University and Mansoura University, Egypt) using dimethyl sulfoxide (DMSO-d₆) or D₂O as a solvent. The chemical shift was estimated in ppm on δ scale and J (coupling constant) was estimated in Hertz. Microanalysis for C, H, and N were performed on a PerkinElmer 2400 analyzer (PerkinElmer, Norwalk, CT. USA) at the regional center for mycology and Biotechnology, Al-Azhar University, Egypt. All results were within ± 0.4% of the theoretical values.