Zen 2.3 sp1 fp3 black 64bit version

In vitro FRAP assays were performed with a Zeiss 880 Confocal Laser Scanning Microscope equipped with a 100×, 1.4 NA oil immersion objective. Samples were prepared as described above. All in vitro FRAP experiments used the 488 nm laser at 100% for bleaching conditions. For N-polyC at 150 mM NaCl, 5 bleaching iterations were used, and images were taken every 2 seconds for 232 seconds. For viral RNA at 150 mM NaCl, 100 bleaching iterations were used, and images were taken every 10 seconds for 8 minutes. For the experiments conducted at 50 mM NaCl, droplets containing polyC RNA or viral RNA were bleached with 20 iterations, and images were taken every 5 seconds for 5 minutes. For droplets containing N protein only, 20 bleaching iterations were used, and images were collected every 2 seconds for 6.5 minutes. All data were acquired using Zeiss Zen 2.3 SP1 FP3 (black) (64bit) Version 14.0.20.201. Data were background corrected, converted to normalized intensity, and fit to an exponential decay function in Origin.