For publicly available data previously published by us, raw sequencing reads were downloaded from NCBI GEO, Study GSE151362 [24 (link)] and combined with new small-RNA dataset with sequencing as follows. miRNA was isolated from 200 μL plasma samples using the Qiagen miRNA serum/plasma kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and stored at −80 °C.
Library preparation and sequencing was performed using Qiagen (Valencia, CA) with the QIAseq miRNA Library and QIAseq miRNA 48 Index IL kits as per the manufacturer’s instructions (Qiagen, Hilden, Germany). Amplified cDNA libraries underwent single-end sequencing by synthesis (Illumina v1.9) using the Illumina NovaSeq 75 bp single-end read sequencing on miRNA libraries from 42 plasma samples taken from 10 non-pregnant and 32 pregnant women aged 16 to 44 years.
Library preparation and sequencing was performed using Qiagen (Valencia, CA) with the QIAseq miRNA Library and QIAseq miRNA 48 Index IL kits as per the manufacturer’s instructions (Qiagen, Hilden, Germany). Amplified cDNA libraries underwent single-end sequencing by synthesis (Illumina v1.9) using the Illumina NovaSeq 75 bp single-end read sequencing on miRNA libraries from 42 plasma samples taken from 10 non-pregnant and 32 pregnant women aged 16 to 44 years.