Immunophenotypical analysis was performed on cryopreserved PBMCs. All antibodies were obtained from BD Biosciences except anti-human IgA (rabbit polyclonal, DakoCytomation), AquaAmine (Invitrogen) and anti-human CCR9 (R&D Systems). The following antibodies were used: anti-CD19 (HIB19), anti-β7 (FIB504), anti-α4 (CD49d, 9F10), anti-CD27 (M-T 271), anti-IgG (G18–145), anti-CD38 (HB7), anti-β1 (CD29, MAR4) and anti-CD3 (SK7). Live and single cells were gated and CD3 + population was excluded. Data were collected on a Becton Dickinson (BD) LSR II cytometer using FACS-DIVA software (BD Biosciences) and analyzed using FlowJo software version Vx 0.6 (Tree Star Inc, San Carlos, CA).
Anti human iga
Manufactured by Agilent Technologies
The Anti-human IgA is a laboratory equipment product designed to detect and quantify the immunoglobulin A (IgA) antibody in human samples. It serves as a tool for researchers and clinicians to analyze the levels of this specific antibody, which plays a crucial role in the immune system.
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Lab products found in correlation
Facsdiva software, by BD (1 mentions)
Lsrii cytometer, by BD (1 mentions)
Streptavidin bv421, by BioLegend (1 mentions)
Igg1 fitc, by BD (1 mentions)
Pe cy7 cd105, by BioLegend (1 mentions)
Ki67 pe cy7, by BD (1 mentions)
Cd31 pe, by BioLegend (1 mentions)
Cd73 biotin clone ty 11, by BioLegend (1 mentions)
Anti mouse iga ab pe, by Abcam (1 mentions)
Pe anti mouse cd138, by BioLegend (1 mentions)
Anti rabbit igg bv421, by BioLegend (1 mentions)
Cd45 percp cy5, by BioLegend (1 mentions)
B220 apc cy7, by BioLegend (1 mentions)
2 protocols using anti human iga
1
Immunophenotyping of Cryopreserved PBMCs
2
Immunostaining and Flow Cytometry Protocols
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The following Abs were used for IF and IHC staining: anti-mouse IgA Ab-PE (Abcam, 97013) or fluorescein isothiocyanate (FITC; Abcam, 97234), anti-mouse CD138-PE (clone 281-2, BioLegend), anti-human IgA (DAKO, #IR51061), or CD138 (DAKO, M7228). For flow cytometry, the following Abs against mouse antigens were used: B220–APC-Cy7 (clone RA3-6B2, BioLegend), CD138-PE or BV421 (clone 281-2), GL7-PerCP Cy5.5 (clone GL7, BioLegend), Ki67–PE-Cy7 (clone B56, BD), IgG1-FITC (clone A85-1, BD), IgG2a/c-FITC (clone RMG2a-62, BioLegend), IgG2b-FITC (SouthernBiotech, 1092-02), IgG3-FITC (SouthernBiotech, 1102-02), IgA-biotin (clone RMA-1, BioLegend), CD73-biotin (clone TY/11.8, BioLegend), CD140b-APC (clone APB5, BioLegend), CD105–PE-Cy7 (clone MJ7/18, BioLegend), CD45–PerCP-Cy5.5 (clone 30-F11, BioLegend), and CD31-PE (clone 390, BioLegend); other reagents were also used: rabbit polyclonal anti-SPTBN1 (amino acids 2100 to 2150; Abcam, 72239), normal rabbit IgG (an isotype-matched control for the latter; Sigma-Aldrich, 12-370), anti-rabbit IgG-BV421 (clone Poly4064, BioLegend), streptavidin-BV421 (BioLegend, 405226), or APC (BioLegend, 405207).
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