After 72 h doxycycline induction-transduced KMS11 cells were fixed in 1 ml ice-cold 70% ethanol for 15 min, and then washed and resuspended in 860 μl PBS, 100 μl RNase (1 mg ml−1; Sigma) and 40 μl PI (1 mg ml−1; Sigma). After incubation at 37 °C for 25 min, cells were analysed on a BS LSRII flow cytometer (BD Bioscience).
Bs lsrii flow cytometer
Manufactured by BD
The BD LSR II flow cytometer is a compact, flexible, and high-performance instrument designed for multi-parameter cell analysis. It features a solid-state air-cooled laser design, high-sensitivity detectors, and advanced optical and electronics components to enable accurate and reliable data acquisition. The BD LSR II is capable of simultaneous detection and analysis of multiple fluorescent and light scatter parameters on single cells or particles.
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Lab products found in correlation
2 protocols using bs lsrii flow cytometer
1
Cell Cycle Analysis of Doxycycline-Induced Cells
2
Immunophenotyping of Embryonic Stem Cells
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EBs were digested into single cells by incubating with 0.25% trypsin (Invitrogen) plus 0.25% collagenase II (Invitrogen, 1:1) for 30 min at 37°C. The isolated single cells (1 × 106) were then immunostained with the antibodies listed below in PBS with 0.2% Tween 20. After cell aggregates were removed, single cells were analyzed on the BS LSRII flow cytometer (BD Bioscience) and analyzed using CellQuest software (BD Biosciences). Polyclonal rabbit IgG isotype control (Abcam, ab171870) was included in all experiments. The following antibodies were used: anti-Tbx5 (Abcam, ab101227), anti-GATA4 (Abcam, ab84593), and Alexa Fluor 488 donkey anti-rabbit IgG (H + L) (Invitrogen, A-21206).
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