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3 4 13c2 cholesterol

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[3,4-13C2]-cholesterol is a stable isotope-labeled compound that contains two 13C atoms at positions 3 and 4 of the cholesterol molecule. It is used as a research tool in various applications, such as metabolic studies, to trace the fate of cholesterol in biological systems.

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Lab products found in correlation

Trimethyl 13c3 caffeine, by Cambridge Isotopes (3 mentions) Formic acid, by Merck Group (3 mentions) 13c6 d glucose, by Cambridge Isotopes (3 mentions) 15n indole, by Cambridge Isotopes (3 mentions) 2 15n l lysine dihydrochloride, by Cambridge Isotopes (3 mentions) 13c7 benzoic acid, by Cambridge Isotopes (3 mentions) 15n l tyrosine, by Cambridge Isotopes (3 mentions) 15n2 uracil, by Cambridge Isotopes (3 mentions) 3 3 13c2 cystine, by Cambridge Isotopes (3 mentions) 1 2 13c2 palmitic acid, by Cambridge Isotopes (3 mentions) 15n choline chloride, by Cambridge Isotopes (3 mentions) 13c5 l glutamic acid, by Cambridge Isotopes (3 mentions) Lc ms water, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) 2 deoxyguanosine 15n2 13c10 5 monophosphate, by Cambridge Isotopes (2 mentions) Methanol, by Merck Group (1 mentions)

3 protocols using 3 4 13c2 cholesterol

1

Isotopic Standards for Mass Spectrometry

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as internal standards [18 (link)], including [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Teeny S., Jarrell Z.R., Krigbaum N.Y., Cirillo P.M., Go Y.M., Cohn B.A, & Jones D.P. (2023). Third Trimester Serum Amino Acid Metabolism is Associated with Maternal Breast Cancer Diagnosed within 15 years of Pregnancy. Research Square.
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2

Comprehensive Metabolite Quantification Protocol

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as an internal standard (Go et al. 2015 (link)) included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2′-deoxyguanosine-15N132, C10-5′-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Go Y.M., Weinberg J., Teeny S., Cirillo P.M., Krigbaum N.Y., Singer G., Tran V., Cohn B.A, & Jones D.P. (2023). Exposome epidemiology for suspect environmental chemical exposures during pregnancy linked to subsequent breast cancer diagnosis. Environment international, 178, 108112.
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3

Targeted Metabolomics Stable Isotope Profiling

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals used as an internal standard [57 (link)] included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Jarrell Z.R., Smith M.R., Hu X., Orr M., Liu K.H., Quyyumi A.A., Jones D.P, & Go Y.M. (2020). Plasma acylcarnitine levels increase with healthy aging. Aging (Albany NY), 12(13), 13555-13570.
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