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4 aminobutyric acid

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4-aminobutyric acid is a chemical compound that is commonly used in various laboratory applications. It serves as a precursor for the synthesis of other compounds and is often employed in biochemical research and analysis.

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Lab products found in correlation

Glycine, by Cambridge Isotopes (2 mentions) Tyramine hcl, by Cambridge Isotopes (2 mentions) L alanine, by Cambridge Isotopes (2 mentions) L phenylalanine, by Cambridge Isotopes (2 mentions)

2 protocols using 4 aminobutyric acid

1

Isotopic Labeling of Bacterial Conjugation with DCA

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To test for conjugation of isotopically labeled amines to DCA by B. fragilis P207, 1 ml cultures containing 1 mM (final concentration) of either 4-aminobutyric acid (13C4, 97-99%), L-alanine (13C3, 99%), glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) was added to BHIS containing 0.1% (w/v) deoxycholic acid (DCA) (Fisher Scientific). Control samples contained either no isotopically labeled amines, no DCA, or BHIS media without any additional supplement. Culture supernatants were prepared for metabolomic analysis as described above.
Mullowney M.W., Fiebig A., Schnizlein M.K., McMillin M., Rose A.R., Koval J., Rubin D., Dalal S., Sogin M.L., Chang E.B., Sidebottom A.M, & Crosson S. (2023). Microbially-catalyzed conjugation of GABA and tyramine to bile acids. bioRxiv.
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2

Tracing Isotope-Labeled Amine Conjugation

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To test for conjugation of isotopically labeled amines to DCA by B. fragilis P207, 1-mL cultures containing 1 mM (final concentration) of either 4-aminobutyric acid (13C4, 97%–99%), L-alanine (13C3, 99%), glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) were added to BHIS containing 0.1% (wt/vol) DCA (Thermo Fisher Scientific). Control samples contained either no isotopically labeled amines, no DCA, or BHIS media without any additional supplement. Culture supernatants were prepared for metabolomic analysis as described above.
Mullowney M.W., Fiebig A., Schnizlein M.K., McMillin M., Rose A.R., Koval J., Rubin D., Dalal S., Sogin M.L., Chang E.B., Sidebottom A.M, & Crosson S. (2024). Microbially catalyzed conjugation of GABA and tyramine to bile acids. Journal of Bacteriology, 206(1), e00426-23.
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